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Start Over Author "Roque Pacheco de Almeida" Publisher universidade federal de uberlandia
2 results on '"Roque Pacheco de Almeida"'

1. Assessment of intracanal medications cytotoxicity on L929 fibroblast cells

Author

Michelle de Paula Farias, Felipe de Souza Matos, Nayane Chagas Carvalho, Roque Pacheco de Almeida, Adriano Augusto Melo de Mendonça, Ricardo Luiz Cavalcanti de Albuquerque Júnior, and Maria Amália Gonzaga Ribeiro

Subjects
cytotoxicity tests, immunologic, endodontics, calcium hydroxide, iodoform, fibroblasts, Agriculture, Biology (General), QH301-705.5
Abstract

This study aimed to evaluate the cytotoxicity of intracanal medications on L929 fibroblast cells at different periods of observation. The following experimental groups were studied: calcium hydroxide with camphorated paramonochlorophenol and glycerin (CPG); iodoform with glycerin (IG); calcium hydroxide with iodoform and distilled water (CIW); iodoform with distilled water (IW); calcium hydroxide with distilled water (CW); Otosporin® (OT); and a control group composed of cells and culture medium. Eluates were prepared from each group and placed in contact with 1 x 105 cells/well for periods of 30 minutes, 12, 24, 48 and 72 hours, 5 and 7 days. After each experimental period, a cytotoxicity test was performed using methyltetrazolium (MTT) and a spectrophotometer at an optical density of 570 nm to analyze cell viability. The ANOVA and Tukey test with a significance level of 5% was used to analyze the data. At 30 minutes and at 12 hours, all groups were equal to the control group. At 24 hours, there was greater cytotoxicity in the IG group than in the control group (P

Published
2016
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2. Assessment of intracanal medications cytotoxicity on L929 fibroblast cells

Author

Maria Amália Gonzaga Ribeiro, Michelle de Paula Farias, Nayane Chagas Carvalho, Felipe de Souza Matos, Ricardo Luiz Cavalcanti de Albuquerque Júnior, Adriano Augusto Melo de Mendonça, and Roque Pacheco de Almeida

Subjects
medicine.medical_specialty, QH301-705.5, 020209 energy, Calcium hydroxide, 02 engineering and technology, Pharmacology, Endodontics, Health Sciencesv, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Statistical significance, fibroblasts, 0202 electrical engineering, electronic engineering, information engineering, medicine, Cytotoxic T cell, Viability assay, immunologic, calcium hydroxide, Biology (General), Cytotoxicity, cytotoxicity tests, Cytotoxicity tests, Chemistry, Agriculture, 030206 dentistry, Fibroblasts, Iodoform, iodoform, endodontics, Distilled water, General Agricultural and Biological Sciences
Abstract

This study aimed to evaluate the cytotoxicity of intracanal medications on L929 fibroblast cells at different periods of observation. The following experimental groups were studied: calcium hydroxide with camphorated paramonochlorophenol and glycerin (CPG); iodoform with glycerin (IG); calcium hydroxide with iodoform and distilled water (CIW); iodoform with distilled water (IW); calcium hydroxide with distilled water (CW); Otosporin® (OT); and a control group composed of cells and culture medium. Eluates were prepared from each group and placed in contact with 1 x 105 cells/well for periods of 30 minutes, 12, 24, 48 and 72 hours, 5 and 7 days. After each experimental period, a cytotoxicity test was performed using methyltetrazolium (MTT) and a spectrophotometer at an optical density of 570 nm to analyze cell viability. The ANOVA and Tukey test with a significance level of 5% was used to analyze the data. At 30 minutes and at 12 hours, all groups were equal to the control group. At 24 hours, there was greater cytotoxicity in the IG group than in the control group (P

Published
2016

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