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Candidiasis or oral candidiasis is a disease most commonly caused by Candida albicans, present in the microbiota as commensals, it is an opportunistic infection being observed mainly in immunocompromised patients. It is estimated that approximately 50% of the healthy population carries Candida species in the oral cavity. In the treatment of mild oral candidiasis, azole or polyene antifungals are highlighted. The adverse effects and cost of conventional antifungal treatment have led to the search for new drugs, one of the alternatives being terpenes. Extracted from essential oils are complex mixtures of various compounds, myrtenol being an example. In this work, the activity of myrtenol against fluconazole resistant Candida strains was verified. Minimum inhibitory concentration, minimum fungicidal concentration, in addition to modulation and association studies (checkerboard method) were performed via the microdilution technique. With the strains tested, myrtenol demonstrated minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) at concentrations of 512 μg/mL, so it has weak antifungal activity and is fungicidal. In the assay of modulation of myrtenol in subinhibitory concentration with the main antifungal agents, there was an increase in the MIC of these drugs. When myrtenol was associated with these antifungal agents (checkboard method), synergistic or indifferent results were observed with fluconazole or amphotericin B, respectively. Myrtenol has weak antifungal activity against the strains tested, but with a fungicidal characteristic, it does not increase the activity of fluconazole and amphotericin B and, in association with conventional antifungal agents, it showed a synergistic or indifferent effect depending on the strain tested. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES A candidíase ou candidíase oral é uma doença causada mais comumente pela Candida albicans, presentes na microbiota como comensais, é uma infecção oportunista sendo observada principalmente em pacientes imunocomprometidos. Estima-se que aproximadamente 50% da população saudável carrega espécies de Candida na cavidade oral. Na terapêutica das candidíases orais leves destacam-se antifúngicos azólicos ou poliênicos. Os efeitos adversos e custo do tratamento dos antifúngicos convencionais tem levado a busca de novos fármacos, uma das alternativas são os terpenos. Extraídos dos óleos essenciais são misturas complexas de vários compostos, sendo o mirtenol um exemplo. Nesse trabalho verificou-se a atividade do mirtenol contra cepas de Candida resistentes ao fluconazol. Foi realizada a concentração inibitória mínima, concentração fungicida mínima, além dos estudos de modulação e de associação (método checkerboard), via técnica de microdiluição. Com as cepas testadas, o mirtenol demostrou a concentração inibitória mínima (CIM) e a concentração fungicida mínima (CFM) nas concentrações de 512 μg/mL, logo este possui fraca atividade antifúngica e esta tem caráter fungicida. No ensaio de modulação do mirtenol em concentração subinibitória com os principais antifúngicos houve aumento da CIM destes fármacos. Quando se associou o mirtenol com estes antifúngicos (método checkboard), observou-se resultados sinérgicos ou indiferentes com o fluconazol ou a anfotericina B, respectivamente. O mirtenol possui fraca atividade antifúngica frente as cepas ensaiadas, porém com característica fungicida, ele não aumenta a atividade do fluconazol e anfotericina B e em associação com antifúngicos convencionais demonstrou efeito sinérgico ou indiferente dependendo da cepa ensaiada.

Diabetic individuals may present oral manifestations due to high glucose levels, that favors the development of more pathogenic biofilms in the presence of microorganisms such as Candida albicans and Streptococcus mutans. The aim of the study was analyze the effect of different glucose levels simulating different salivary conditions in C. albicans (ATCC 90028) and S. mutans (UA159) duoespecies biofilms in acrylic resins. Initially, a salivary film was developed and standardized or absorbed 600nm. Then, the biofilm cells were cultured for 96 hours on acrylic resin samples. They were subjected to constant exposures of glucose equivalent to 0, 20, 60 and 100 mM salivary levels and were in contact with 10% sucrose three times daily. Colony forming units (CFU / mL), soluble and insoluble extracellular polysaccharides (SEPS and IEPS), biomass, total protein (TP), phospholipase activity and fluorescence morphology analysis were performed. The acidogenicity of the culture medium was also measured. Data were analyzed by ANOVA / Tukey and Kruskal-Wallis / Mann-Whitney (α= 5%). The CFU / mL result for C. albicans determines media between 8.50 ± 0.23 and 8.97 ± 0.24, with significant difference between groups p 0.05). Regarding PECs, wet weight and phospholipase production without differences (p> 0.05), despite the high proteolytic activity. For IEPS, there were differences between the 20 and 60 mM groups (p 0,05). Em relação aos PECs, peso úmido e à produção de fosfolipases não houve diferenças (p>0,05). Para os PECis, houve diferenças entre os grupos de 20 e 60 mM (p

Objective: To evaluate the antifungal potential of 2-Bromo-N-phenylacetamide (A1Br) against oral cavity C. glabrata; Materials and methods: An in vitro laboratory study using an inductive approach was performed. Assays to evaluate the antifungal activity of A1Br, nystatin and miconazole against C. glabrata strains were performed using microdilution technique. Minimum Inhibitory (MIC), and Minimum Fungicide (MFC) Concentrations were determined, as well as evaluation of yeast structures and association tests of the molecule with standard antifungals. Results: The A1Br molecule presented an MIC of 16 μg/mL and an MFC of 32-64 μg/mL; revealing antifungal potential similar to nystatin (MIC 8 μg/mL and MFC 8- 32 μg/mL) and superior to miconazole (MIC 64 g/mL and MFC of 512-1024 g/mL). The A1Br - nystatin association presented antifungal effect. The A1Br - miconazole association presented fungistatic effect. Micromorphological evaluation revealed decreases in all structures for the two studied strains at the antifungal concentration of A1Br =MIC x 2, reaching 100% at A1Br = MIC x 4. The association study with nystatin revealed antagonism (FICI = 4.5) and with miconazole revealed indifference (FICI = 1.25). Conclusion: The studied molecule presents excellent antifungal effect against C.glabrata. Clinical Relevance: The search for new products and formulations is based on a great necessity for more alternatives against the fungi which cause oral C.glabrata infections. Despite the excellent antifungal activity potential of the studied molecule, further studies are needed to evaluate mechanisms of action and associations with other substances. Nenhuma Objetivo: Avaliar o potencial antifúngico de derivado de acetamida 2-Bromo-Nfenil acetamida (A1Br) contra Candida glabrata oriundas da cavidade bucal. Materiais e métodos: Realizou-se um estudo in vitro laboratorial, com abordagem indutiva. Os ensaios para avaliação da atividade antifúngica de A1Br,nistatina e miconazol contra linhagens de C. glabrata foram realizados pela técnica da microdiluição. Foram determinadas as Concentrações Inibitórias Mínimas (CIM), Fungicida Mínima (CFM) bem como avaliação das estruturas leveduriformes e testes de associação da molécula com os antifúngicos licenciados. Resultados: Observou-se que a molécula A1Br obteve CIM de 16 g/mL e CFM de 32-64g/mL apresentando potencial antifúngico similar a nistatina(CIM 8g/mL e CFM entre 8-32g/mL) e superior ao miconazol(CIM 64 g/mL e CFM entre 512-1024g/mL) obtendo A1Br e nistatina efeito fúngicida e miconazol fungistático. Na avaliação micromorfologica houve diminuição de todas as estruturas de A1Br nas concentrações CIM x 2 e antifúngicos com redução parcial na mesma para as duas linhagens estudadas,chegando a 100% na concentração de CIM x 4, teste Mann-Whitney em comparação com o controle (P < 0,05). No estudo de associação apresentou antagonismo para nistatina (ICIF = 4,5) e indiferença para o miconazol (ICIF = 1,25). Conclusão: A molécula estudada possui ótimo efeito antifúngico frente a C.glabrata. Relevância clínica: Houve uma busca de novos produtos para formulações baseados na necessidade de mais alternativas contra fungos causadores infecções orais por C.glabrata. Apesar do ótimo potencial de ação antifúngico da molécula estudada se faz necessário mais estudos para avaliar mecanismos de ação desse produto e associação com outras substâncias.

Introduction: Oral candidiasis represents one of the greatest challenges to be faced by dentistry, either because of the limited number of antifungal agents available or because of the expressive resistance presented by microorganisms involved in this type of infection. Objective: This study evaluated the effectiveness of a mouthwash and a spray containing essential oil (EO) extracted from leaves of Cinnamomum zeylanicum Blume (cinnamon) in the treatment of bucal candidosis associated with the use of dental prosthesis. Methodology: A randomized, double blinded, controlled clinical trial was conducted in a probabilistic sample of 36 participants with the disease, divided into two groups that used the experimental solution (C. zeylanicum, n = 18) and control (nystatin, n = 18). Mycological and clinical parameters were evaluated, by a calibrated examiner, before and after 15 days of use of the products. A count of the Colony Forming Units (CFU) of Candida spp. of the biological samples collected with a swab on the palate and on the prosthesis of the patients was done before and after treatment. Data were analyzed by descriptive and inferential statistical analyzes, based on mycological and clinical parameters, considering α = 5%. Results: The clinical examination of the affected mucosa showed that the products containing C. zeylanicum EO (p = 0.0339) and nystatin (p = 0.0139) promoted clinical efficacy, with significant reduction of signs and symptoms observed. The mycological analysis expressed by the number of CFU indicated that the EO of C. zeylanicum had no significant effect (p> 0.05) on the reduction of Candida spp. isolated from the mucosa and dental prosthesis. Nystatin, however, promoted a significant reduction in the colonization of Candida spp. in the mucosa (p 0,05) na redução de Candida spp. isoladas da mucosa e prótese dentária. A nistatina, porém, promoveu redução significativa da colonização de Candida spp. na mucosa (p

This work was aimed at the evaluation of the antifungal action of isolated and associated (+)-α-pinene and (+)-β-pinene enantiomers on Candida species, their possible mechanisms of action, their molecular interactions with enzymes related to wall formation and fungal cell membrane, as well as its antibiofilm activity. It was used 25 strains of Candida, of clinical origin and of reference, sensitive and resistant to licensed antifungal agents, for determination of Minimum Inhibitory Concentration (MIC), Minimum Fungicide Concentration (CFM), Fraction Inhibitory Concentration Index (ICIF), Microbial Time-kill Curve, possible mechanisms of action, molecular docking and the effect on the inhibition of the biofilm adherence provided by the enantiomers. A descriptive and inferential statistical analysis was performed, considering α=5%. The MIC values ranged from 114.06µM to 7300µM (15.6 µg/mL to 1000µg/mL) and from

The Candida genus usually has a commensal relationship in the oral cavity, modifying factors may alter this relationship directly or indirectly, making it pathogenic, triggering oral candidiasis. The phytotherapeutic products have been constituting a therapeutic alternative, widely used in the treatment of candidiasis. The objective of this study was to isolate and identify strains of Candida tropicalis and Candida parapsilosis from the oral cavity of healthy individuals through macro and micromorphology, physiological and biochemical tests. The in vitro and in silico sensitivity of these strains to eugenol and nystatin was then evaluated by determining the Minimum Inhibitory Concentration (MIC); Minimal Fungicide Concentration (MFC) and analyzing the mechanisms of action through microbial growth kinetics, micromorphology and association study (checkboard), and in silico assay. Eight strains of Candida tropicalis, one from the American Type Culture Collection - ATCC 13803 and seven from clinical origin and seven from Candida parapsilosis to their ATCC standard (22019) and six from clinical isolates were used. An antifungal activity of eugenol on the tested species was observed, a MIC and MFC of 64 μg / mL for Candida tropicalis was determined. The CFM / MIC ratio indicates fungicidal activity compared to 87.5% of the tested strains. In the Candida parapsilosis species the MIC was 256 μg / mL which coincided with the CFM in 100% of the strains tested. Regarding the association assay, strain ATCC 13803 and another clinic - LM-34 of C. tropicalis were used, the results showed antagonism of the substances analyzed for both the ATCC 22019 strain and the LM-34 clinical strain. Micromorphology demonstrated inhibition of virulence structures such as pseudohyphae and blastoconideas at the three concentrations analyzed for C. tropicalis (ATCC 13803 and LM-34) and C. parapsilosis (ATCC 22019 and LM-70) strains. The microbial death curve showed a fungicidal effect after 24 hours of experiment in both species studied. The results of the present study indicate a strong activity of the eugenol molecule on the strains of Candida tropicalis and Candida parapsilosis analyzed and could represent a therapeutic alternative for the treatment of oral candidiasis. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES O gênero Candida normalmente possui uma relação de comensalismo na cavidade bucal, fatores modificadores podem alterar essa relação de forma direta ou indiretamente, tornando-o patogênico, desencadeando a candidíase. Os produtos provenientes de plantas medicianais vêm constituindo uma alternativa terapêutica, amplamente utilizada no tratamento da candidíase. Objetivou-se isolar e identificar cepas de Candida tropicalis e Candida parapsilosis da cavidade bucal de indivíduos saudáveis, através da macro e micromorfologia, provas fisiológicas e bioquímicas. Avaliou-se a sensibilidade in vitro dessas cepas frente ao eugenol e a nistatina, através da determinação da Concentração Inibitória Mínima (CIM); Concentração Fungicida Mínima (CFM) e curva de morte, micromorfologia e estudo de associação (checkboard). Utilizaram-se oito cepas de Candida tropicalis, uma cepa padronizada (ATCC 13803) e sete de origem clínica e seis cepas clínicas de Candida parapsilosis e sua padrão (ATCC 22019). Observou-se atividade antifúngica do eugenol sobre as espécies ensaiadas, sendo determinada a CIM/CFM de 64 μg/mL para Candida tropicalis. A relação CFM/CIM indica atividade fungicida frente a 87,5% das cepas testadas. Na espécie de Candida parapsilosis a CIM foi de 256 μg/mL a qual coincidiu com a CFM em 100% das cepas ensaiadas. Com relação ao ensaio de associação, utilizou-se a cepa ATCC 13803 e outra clínica - LM-34 de C. tropicalis. Os resultados mostraram antagonismo das substâncias analisadas tanto para cepa ATCC 22019, quanto para cepa clínica LM-34. O estudo da micromorfologia demonstrou inibição das estruturas de virulência como pseudo-hiifas e blastoconídeos nas três concentrações analisadas (CIM; CIMx2 e CIMx4), em ambas as cepas avaliadas de C.tropicalis e C. parapsilosis. A curva de morte microbiana mostrou um efeito fungicida após 24 horas de experimento em ambas as espécies estudadas. Os resultados do presente estudo indicam uma forte atividade da molécula do eugenol sobre as cepas de Candida tropicalis e Candida parapsilosis analisadas, podendo representar uma alternativa terapêutica para o tratamento da candidíase oral.

Oral candidiasis is a fungal infection caused by yeasts of the genus Candida, with C. albicans being the main pathogen. The emergence of resistant species against the available therapeutic arsenal as well as its adverse effects has driven new research on natural products. In view of this problem, the present study sought to investigate the antifungal activity of essential oil (OE) obtained from the leaves of Melissa officinalis L (lemon balm), isolated and associated with antifungal agents licensed on C. albicans strains. Two strains of the American Type Culture Collection (ATCC) and eight from the oral cavity (LM) were selected. The microdilution and sowing of subcultures were used to determine, respectively, the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (CFM). The antifungigram was performed using the disc diffusion technique with the antifungal agents nystatin, amphotericin B, miconazole and fluconazole, evaluating them in isolation and associated with OE. MIC and MFC ranged from 64 to 128 μg/mL and 256 to 512 μg/mL, respectively. The presence of resistance of the strains to the antifungal agents nistatin (LM-38 and LM-65), amphotericin B (ATCC-76485, LM-3A, LM-38, LM-62 and LM -65) and fluconazole (LM-1A, LM- 1B, LM-38, LM-62 and LM-65). In the association tests, the results showed the influence of the essential oil on the antifungal activity, with synergism (S), indifference (I) and antagonism (A) being observed. The percentages found for each antifungal in the order (S, I and A) were: nystatin (30%, 30% , 40%), amphotericin B (30%, 50%, 20%), fluconazole (20% ,40% , 40%) and miconazole (100%, 0,0). In view of the above, it is concluded that the OO of M. Officinalis may become a promising product in the fight against oral candidiasis, since it has a strong antifungal activity against Candida albicans and that the use of this product associated with the licensed antifungal can, in some situations, potentiate its effectiveness of use in treatment. A candidíase oral é uma infecção fungica causada por leveduras do gênero Candida, sendo a espécie C. albicans apontada como principal patógeno. O surgimento de espécies resistentes frente ao arsenal terapêutico disponível assim como seus efeitos adversos tem impulsionado novas pesquisas voltadas para os produtos naturais. Diante dessa problemática o presente trabalho buscou investigar a atividade antifúngica do óleo essencial (OE) obtido das folhas de Melissa officinalis L (erva-cidreira), isolado e associado a antifúngicos licenciados sobre cepas de C. albicans. Para tanto, foram selecionadas duas cepas da American Type Culture Collection (ATCC) e oito provenientes de cavidade oral (LM). Utilizou-se a técnica da microdiluição e semeadura de subcultivos para determinar, respectivamente, a Concentração Inibitória Mínima (CIM) e a Concentração Fungicida Mínima (CFM). O antifungigrama foi realizado a partir da técnica de difusão de disco com os antifúngicos nistatina, anfotericina B, miconazol e fluconazol avaliando-os de forma isolada e associada ao OE. A CIM e CFM variaram, respectivamente, de 64 a 128 μg/mL e 256 a 512 μg/mL. Quanto ao teste de sensibilidade de forma isolada observou-se a presença de resistência das cepas aos antifúngicos nistatina (LM-38 e LM-65), anfotericina B (ATCC- 76485, LM-3A, LM-38, LM-62 e LM-65) e fluconazol (LM-1A, LM-1B, LM-38, LM- 62 e LM-65). Nos ensaios de associação os resultados mostraram influência do óleo essencial sobre a atividade dos antifúngicos sendo observado sinergismo (S), indiferença (I) e antagonismo (A). As porcentagens encontradas para cada antifúngico, na ordem (S, I e A), foram: nistatina (30%, 30% e 40%), anfotericina B (30%, 50%, 20%), fluconazol (20%, 40% e 40%) e miconazol (100%,0,0). Perante o exposto conclui-se que o OE de M. Officinalis pode se tornar um produto promissor no combate a candidose oral, visto que apresenta forte atividade antifungica contra Candida albicans e que o uso desse produto associado à antifungicos licenciados pode, em algumas situações, potencializar sua efetividade de uso no tratamento.

Introduction: The phytotherapy has encouraged the evaluation of different herbal products activity to control dental problems, by creating new strategies for the chemical control of oral cavity infections, in an attempt to overcome the drawbacks and weaknesses of market products. Objective: To isolate, identify Candida genus yeasts from denture wearers (removable partial dentures or complete dentures) and evaluate the in vitro antifungal activity of an essential oil extracted from the leaves of Schinus terebinthifolius Raddi. Materials and Methods: It was collected biological material from 23 prosthesis wearers and the identification of yeasts was based on macro and micro-morphology, physiological and biochemical tests. In the antifungal activity tests of S. terebinthifolius Raddi was used four strains of Candida albicans (C. albicans LM-1A, C. albicans LM-3A, C. albicans LM-16B, C. albicans LM-19A), four strains of Candida tropicalis (C. tropicalis LM-2A, C. tropicalis LM-7A, C. tropicalis LM-11A, C. tropicalis LM-13A) of yeasts isolated and identified by biological material collect and standard strains of the american collection ATCC (American Type Culture Collection): C. albicans ATCC 76485 and C. tropicalis ATCC 13803. The sensitivity profile to synthetic antifungal was evaluated by diffusion technique in solid medium suitable for antifungal susceptibility test. The antifungal agents used were amphotericin B (100 μg), fluconazole (25 μg), itraconazole (10 μg), miconazole (50 μg), nystatin (100 I.U.), ketoconazole (50 μg). It was determined the minimum inhibitory concentration (MIC) of essential oil by the microdilution technique and calculated the minimum fungicidal concentration (MFC) by subculture on Sabouraud Dextrose Agar (SDA). Results: Most of the denture wearers were female (78%) and the average age was 48 years and two months. C. albicans and C. tropicalis were the most prevalent species in samples taken in the palatal mucosa (17.39% each) and the bases of prostheses (21.73% each). The antifungal that all strains tested were sensitive to was amphotericin B followed by nystatin, ketoconazole and miconazole with respectively 90%, 80%, 50% of the susceptible strains. Most strains (90%) were resistant to fluconazole and all were to itraconazole. The MIC values varied from 128μg/mL to 256μg/mL, being determined the MIC100% as 256μg/mL. For nystatin, the MIC100% was 16μg/mL. The MFC/MIC ratio indicates fungicidal activity against 80% of the strains tested and is considered fungistatic against C. tropicalis LM-7A and C. tropicalis LM-13A. For nystatin, indicated fungicidal activity against all tested strains. Conclusion: The most prevalent species in denture wearers were C. albicans and C. tropicalis, with a varied susceptibility profile compared to synthetic antifungal, highlighting a pattern of resistance of some strains, particularly to fluconazole and itraconazole. The essential oil of S. terebinthifolius Raddi showed fungicidal activity against most of the strains tested. Introdução: A fitoterapia tem estimulado a avaliação da atividade de diferentes produtos à base de plantas para o controle de problemas bucais, visando criar novas estratégias para o controle químico das infecções da cavidade bucal, na tentativa de suprir os incovenientes e fragilidades dos produtos do mercado. Objetivo: isolar, identificar leveduras do gênero Candida da cavidade bucal de usuários de prótese (parcial e total removível) e avaliar a atividade antifúngica in vitro do óleo essencial extraído das folhas de Schinus terebinthifolius Raddi (aroeira). Materiais e Métodos: Foram coletados material biológico de 23 usuários de prótese e a identificação das leveduras foi realizada com base na macro e micromorfologia, provas fisiológicas e bioquímicas. Para os ensaios da atividade antifúngica de S. terebinthifolius Raddi foram utilizadas quatro cepas de Candida albicans (C. albicans LM-1A, C. albicans LM-3A, C. albicans LM-16B, C. albicans LM-19A), quatro cepas de Candida tropicalis (C. tropicalis LM-2A, C. tropicalis LM-7A, C. tropicalis LM-11A, C. tropicalis LM-13A) de leveduras isoladas e identificadas da coleta do material biológico e cepas padrão da coleção americana ATCC (American Type Culture Collection) C. albicans ATCC 76485 e C. tropicalis ATCC 13803. O perfil de sensibilidade aos antifúngicos sintéticos foi avaliado pela técnica de difusão em meio sólido indicada para antifungigrama. Os antifúngicos utilizados foram anfotericina B (100 μg), fluconazol (25 μg), itraconazol (10 μg), miconazol (50μg), nistatina (100 U.I.), cetoconazol (50μg). Foi determinada a concentração inibitória mínima (CIM) do óleo essencial e da nistatina (controle) pela técnica da microdiluição e calculada a concentração fungicida mínima (CFM) através do subcultivo em Agar Sabouraud Dextrose (ASD). Resultados: A maioria dos usuários de prótese eram do sexo feminino (78%) e a média de idade de 48 anos e dois meses. C. albicans e C. tropicalis foram as espécies mais prevalentes nas coletas realizadas nas mucosas palatinas (17,39% cada) e nas bases das próteses (21,73% cada). O antifúngico que todas as cepas testadas apresentaram-se sensíveis foi a anfotericina B, seguida da nistatina, cetoconazol e miconazol com respectivamente 90%, 80%, 50% das cepas sensíveis. A maioria das cepas (90%) foram resistentes ao fluconazol e todas foram ao itraconazol. Os valores da CIM do óleo essencial variaram entre 128μg/mL (CIM60%) e 256μg/mL (CIM100%). Para a nistatina a CIM100% foi de 16μg/mL. A relação CFM/CIM do óleo essencial indica atividade fungicida frente a 80% das cepas testadas, sendo considerada fungistática diante da C. tropicalis LM-7A e C. tropicalis LM-13A. E a relação CFM/CIM da nistatina indica atividade fungicida frente a todas as cepas estudadas. Conclusão: As espécies mais prevalentes nos usuários de prótese foi C. albicans e C. tropicalis, com perfil de sensibilidade variado em relação aos antifúngicos sintéticos, com destaque para um padrão de resistência de algumas cepas, especialmente ao fluconazol e itraconazol. O óleo essencial de S. terebinthifolius Raddi apresentou atividade fungicida sobre a maioria das cepas testadas.

Introduction: Faced with limitations to the use of sodium hypochlorite in the disinfection of dental prostheses and the need to control fungal proliferation in these sites, it is necessary to study new substances for this purpose. Objectives: To evaluate the antifungal activity, mechanism of action, cytotoxicity and antibiofilm action of chloramine T (CAT) on Candida spp. The minimum inhibitory concentration (MIC) of the substance on Candida albicans, Candida tropicalis, Candida krusei and Candida glabrata was determined by the microdilution technique and the minimum fungicidal concentration (CFM) was calculated through the subculture in Sabouraud Dextrose Agar (ASD) . The growth inhibition kinetics of C. albicans were evaluated by the method of counting colony forming units (CFU) at different times and concentrations. A microculture of C. albicans was performed on fowl agar plus tween 80 to evaluate the possible alteration of micromorphology against different concentrations of the substance. The possible mechanism of action on wall and fungal cell membrane was verified by the determination of MIC in the presence of sorbitol and ergosterol respectively. The inhibition of the initial adherence of fungal cells, formation and reduction of C. albicans biofilm were evaluated after short (1 min) and prolonged (8 h) contact with the substance and formation of the biofilm was measured by absorbance at 600 nm, Transformed into scores referring to the percentage of inhibition obtained based on the values ​​of the control group. The cytotoxicity of the substance was evaluated by the hemolysis method. Nystatin and sodium hypochlorite were used as positive controls. A descriptive and inferential analysis was performed considering α = 5%. Results: The CIM75% found for CAT was 781.3 μg / mL and the CFM / MIC ratio suggests a fungicidal activity against most of the strains tested, with probable action on cell wall and membrane. The substance showed immediate and prolonged action on the kinetic test and caused reduction of the filamentous form and inhibition of chlamydoconidia. In the biofilm assay, it presented similar results to sodium hypochlorite for inhibition of initial adherence and formation of mature biofilm (p> 0.05) and was more effective in reducing mature biofilm in the short contact groups at MIC concentration x2 (24 H) and CIM x 4 (48 h) (p 0,05) e foi mais efetiva na reducao do biofilme maduro nos grupos de contato curto na concentracao CIM x 2 (24 h) e CIM x 4 (48 h) (p < 0,05). Conclusao: A CAT apresenta atividade antifungica sobre Candida spp. e apresenta acao fungicida sobre a maioria das cepas testadas. Sua acao e imediata e prolongada na inibicao do crescimento de C. albicans e provavelmente ocorre tanto em parede quanto em membrana celular. A CAT causa alteracoes na micromorfologia de C. albicans e possui atividade antibiofilme, sendo efetiva na inibicao da aderencia inicial das celulas fungicas, bem como na formacao e reducao do biofilme.