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In the present work an analytical methodology to micr o scale based on the use of the HS - SPME/GC - MS to determine volatile compounds present in Clinopodium odorum (Griseb.) Harley (Lamiaceae)was optimized and settled differences and similarities with its essential oil. A systematic description of the volatile components of flowers, stems, leaves and combined aerial parts (whole plant) was constructed via GC - MS analyses of HS - SPME adsorbed compounds and of essential oils obtained through hydrodistillation of the same tissues. Pulegone was the main component of b oth the HS - SPME analysis and essential oil analysis. In addition, piperitenone oxide and piperitone oxide were the other main components in the essential oil whereas in the HS - SPME analysis cis - isopulegone and menthone prevailed. The HS - SPME method can ach ieve comparable results to those obtained by essential oil analysis, by using very fewer samples, a shorter extraction time and a much simpler procedure., Fil: Vázquez, Ana María. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina, Fil: Aimar, Mario Leandro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina, Fil: Demmel, Gabriela Inés. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina, Fil: Cabalén, María Eugenia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina, Fil: Decarlini, María Florencia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina, Fil: Cantero, Juan. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina, Fil: Criado, Silvia Gertrudis. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina, Fil: Ruiz, Gustavo M. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; Argentina

A headspace solid-phase microextraction (HS-SPME) method followed by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detection (GC–FID) are described for the analysis of volatile compounds in Tagetes filifolia Lag. The composition of the total aerial parts of the plant (whole plant) and the inflorescences, leaves and stems were studied separately using HS-SPME. As a result, 54 compounds were determined, of which 47 were identified. The major components observed in this analysis were trans-anethole and estragole. The HS-SPME method used for the analysis of volatile compounds of T. filifolia is simple, fast, effective, free from the use of solvents, and permits by an analysis of small amounts of samples to achieve better results in terms of the determination of the composition than those reported in the literature for the analysis of essential oil., Fil: Vázquez, Ana María. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina, Fil: Aimar, Mario L. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina, Fil: Demmel, Gabriela Inés. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina, Fil: Cabalén, María Eugenia. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina, Fil: Criado, Silvia Gertrudis. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina, Fil: Cantero, Juan. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina, Fil: Velasco, Manuel I. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina, Fil: Rossi, Laura I. niversidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina