Search

Background: Jatropha (Jatropha curcas) or physic nut is a potential source for the production of biodiesel as well as its use in the pharmaceutical, agricultural, cosmetic and food industries. Genetic variability is of great importance for future selection of elite plants. Objective: Analyze the genetic diversity of wild Jatropha curcas in Northwest México. Methodology: Young leaves of J. curcas were collected in three different ecotypes in Northern of Sinaloa (El Quelite, Estación Dimas and La Campana). Their diversity was analyzed with 5 primers ISSRs. Results: The ISSR analysis of 27 J. curcas accessions showed an expected heterozygosity (He) and observed heterozygosity (Ho) elevated among in La Campana (He 0.7524, Ho 0.644), Estación Dimas (He 0.7092, Ho 0.5106) and El Quelite (He 0.7956, Ho 0.6660). Implications: The analysis of the genetic variability of wild Jatropha curcas from Sinaloa contributes to be the bases for possible selection of genetic material. Conclusions: The ISSR analysis showed that there is genetic diversity in the wild germplasm of the different regions of Sinaloa, this is important in the selection of plants and the establishment of potential crops for the production of biodiesel, as well as the possibility of improving and identifying new varieties.

Background: Aspergillus parasiticus is a mold to produce aflatoxins, and growth in maize; the used synthetic fungicides for their inhibition are risk to health and the environment. However, garlic essential oil to have antifungal activity and can be alternative for control A. parasiticus. Objective/hypothesis: Essential garlic oil can inhibit the in vitro growth of A. parasiticus and its aflatoxins. Methodology: The in vitro antifungal activity of garlic (Allium sativum L.) essential oil was evaluated against A. parasiticus (radial growth and spore germination). In addition, the mean inhibitory concentrations (IC50) and minimum inhibitory (MIC) were calculated. Aflatoxins were tested on maize with High-Performance Thin-Layer Chromatography (HPTLC). Results: The mean inhibitory concentration (IC50) and minimum inhibitory (MIC) obtained by Probit analyses with radial growth were 971 and 2875 ppm of garlic essential oil, respectively, in A. parasiticus. These concentrations inhibited spore germination until 24 h. Starting from 100 ppm of garlic essential oil, mycelial inhibition was shown. Evidence of inhibition of aflatoxin production by A. parasiticus in maize was not possible at concentrations of 971 and 2875 ppm garlic essential oil. Implications: The concentrations 971 and 2875 ppm of garlic essential oil inhibited the growth in vitro of A. parasiticus. However, they did not inhibit aflatoxin production by A. parasiticus into maize. Conclusion: Garlic essential oil inhibit the in vitro growth of A. parasiticus on growth radial and germination spores; but not inhibit aflatoxins production on maize to concentrations 971 and 2875 ppm of garlic essential oil.