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Analysis of marine environment, especially the multi-scale analysis is an efficient means to obtain the environmental situation and guide the environmental management. In this paper, the field data, max, min and average of dissolved inorganic nitrogen (DIN) and phosphate in the Caofeidian marine district, Tangshan Bay is analyzed. The whole water quality situation could be grasped through these analyses. The research results show that the distribution of pollution is disequilibrium and the high value district of pollution exists in research district too.

The correlation analysis of pollution time series is an essential tool for obtaining the relationship in adjacent marine waters and the source of pollution. In this paper, the correlation of the dissolved inorganic nitrogen (DIN) in the Caofeidian marine district and the Beidaihe marine district, Tangshan Bay is analyzed. The spearman correlation coefficient computation method and the least squares fitting method are used for the correlation and the trend respectively. The research results show that the trend of DIN is on the rise in recently. Simultaneously, some certain correlation exists in the Caofeidian marine district and the Beidaihe marine district, owing to the adjacent characteristics and synchronous development in this district.

Marine environment is an essential aspect for the regional sustainable development. The distribution and trend of pollutant factors is a key guidance to environmental management. In this paper, the distribution and trend of dissolved inorganic nitrogen (DIN) in marine environment waters is researched for guiding and improve the environmental impact assessment in the Caofeidian marine district, Tangshan Bay. The natural neighbor grid method and the least squares fitting method are used for the distribution and the trend respectively. The research results show that the part of DIN exists exceeded forth level of water quality and the trend of DIN is on the rise in recently.

Heavy metals are important marine environmental factors for the safety and regional sustainable development. In this paper, the Caofeidian marine district in Tangshan Bay is considered as the case for the environmental research. The distribution and trend of heavy metal Pb in marine waters and sediment is analyzed by the natural neighbor grid method and the least squares fitting method. The enriched characteristic of Pb in different marine medium is studied by the trend of Pb in waters and the sediment. The research results show that the part of Pb exists exceeded forth level of water quality in 2007 and the trend of Pb in sediment is on the rise, oppositely in waters. The enriched characteristic analysis points out the object of environmental management.

Strain Gluconacetobacter hansenii CGMCC1671 and Saccharomyces cerevisiae CGMCC1670 were applied to make traditional Kombucha with pure cultures to search for the optimum parameters of major factors affecting the yields and productivities of Bacterial cellulose (BC) in the beverage. Three culture factors were examined. The yields and productivities of BC and sugar consumed were measured after cultured statically for 22 days. After single factor test factors affecting the yields and productivities of BC have been optimized by response surface methodology (RSM). The quadratic polynomial regression equation reflecting BC yield and affecting factors was build up with Box-Behnken design principle. The optimal values of 10.37% inoculum, initial pH 4.96 and medium volume 77.13 mL in 250 mL flask were obtained with theoretical BC yield 300.093mg/g. BC yield of 279.579 mg/g was obtained with 6.84% deviation by validation test with the optimal parameters. The co-culture of pure strains of traditional Kombucha technique can be used to provide both high quality and high yield of BC in addition to producing high quality Kombucha beverage.

For researching ecological behaviors of Cupriavidus taiwanensis X1 which has strong hydrolysis activity on chlorpyrifos(CP), reporter gene luxAB was successfully introduced into cells by electroporation. The labeled strain X1-lux with genetic stability and fluorescence was obtained. Both of strain X1 and X1-lux could completely degrade 200 mg/l CP within 12h in minimal salt medium, and experimental results showed that introduction of luxAB did not affect strain X1 growth and degradation on CP. The cells of X1-lux and X1 were inoculated into soil with 500 mg/l CP, the cell concentration and CP residual was detected, and data revealed that strain X1 could absolutely removed CP in 12d and survive in soil. Strain X1 is a potential excellent choice for bioremediation of organophosphorus polluted environments.

In existing multi-population multi-objective cultural algorithms, information are exchanged among sub-populations by individuals. However, migrated individuals can not reflect the evolution information enough, which limits the evolution performance.In order to enhance the migration efficiency, a novel multi-population multi-objective cultural algorithm adopting knowledge migration is proposed. Implicit knowledge extracted from the evolution process of each sub-population directly reflects the information about dominant search space. By migrating the knowledge among sub-populations at the constant interval, the algorithm realizes more effective interaction with less communication cost. Taken benchmark functions as the examples, simulation results indicate that the algorithm can effectively obtain the Pareto-optimal sets of multi-objective optimization problems. The distribution performance is also improved.