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Experiment disodium phenyl phosphate as a reaction basic, stromal hydrolysis produce phenol, the amount of phenol indirectly indicates the activity of Alkaline Phosphatase.Experiment by absorption wavelength, activated sludge volume, disodium phenyl phosphate, aluminum sulfate, reagent, temperature and other factors affecting the determination of Alkaline Phosphatase were studied.The experiments optimal reaction conditions initially identified. At 37°C, when activated sludge volume of 5mL, 15mL disodium phenyl phosphate absorbance measured at the maximum value, highest levels of Alkaline Phosphatase. Results show that, 0.25mL reagent optimum dosage for the trial, the best absorption wavelength through fitting the peak-valley is 590nm.

The structure-based pharmacophore (SBP) model is consisted by the complementarity of the chemical features and space of the interaction between the ligand and receptor. The SBP models always have a high specificity which can only represent the specific class of the ligand. To simplify the models, sub-pharmacophore was then proposed in present study, and was expected to have and only have the most important or the common chemical features which play the major role in the interaction of ligand and receptor. Sub-pharmacophore should contain 4-6 features, the higher specificity with more features, and vice versa. The sub-pharmacophore was generated by the random combination of features from the structure-based models. With the MDL Drug Data Report database used as the testing database, a new metric CAI (comprehensive appraisal index), which integrated the metrics of E and A%, was defined and used to determine the best sub-pharmacophore model. C-Jun N-terminal kinase (JNKs) is one of the mitogen-activated protein kinase family, and widely involved in immune response and inflammatory response, and other pathological processes. JNK3 is mainly distributed in the brain and nervous system. In present study, twenty-five initial SBP models of JNK3 inhibitors were directly constructed from the Protein Data Bank (PDB) complexes by the LigandScout software. Then, 1018 sub-pharmacophore models were obtained from the 25 initial models. Finally, the best sub-pharmacophore was determined which was simplified from the initial model generated from the 3FI2 complex, and included four features: one hydrogen bond donor, one hydrogen bond acceptor, and two hydrophobic groups. The metrics of E, A% and CAI value of the best sub-pharmacophore model are 17.47, 31.15 and 5.44, respectively. The potential JNK3 inhibitors were then identified from Chinese herbs with the best sub-pharmacophore model, and 286 compounds were obtained.

Multiple targets which closely related to Alzheimer's disease (AD) pathogenesis were selected for pharmacophore models generation and virtual screening in Chinese herbs. The targets comprised Acetylcholinesterase (AchE), muscarinic receptor 1 (M1), γ-secretase and glycogen synthase kinase 3β (GSK-3β). The pharmacophore models, which of AchE inhibitors, M1 agonists, γ-secretase inhibitors and GSK-3β inhibitors, were constructed by distance comparison method. Four testing databases for the evaluation of pharmacophore models were constructed with the active compounds with clearly marked activity on each target. The metric CAI (Comprehensive Appraisal Index) was then used to evaluate and obtain the best pharmacophore models of each target, which were then applied to screen the Traditional Chinese Medicine Database for potential active compounds in Chinese herbs. Four common used herbs were obtained, which contain the active compounds and can act on multiple targets, and were expected to have multiple activity of anti-AD disease.

The combustion process of polymer is a complex coupling of energy feedback from flame to fuel surface with degradation of polymer. In this paper, a series of comparative laboratory-scale experiments were carried out to study the effect of sample width on downward flame spread over the rigid polyurethane (RPU) foam, a typical thermal insulation material. The variations of some important parameters such as flame height, pulsation frequency and flame spread rate were measured and analyzed. Results show that if the width fixed, the parameters mentioned above independent with time. If the width less than 25cm, the sample can not burn completely. With the expand of width, average flame height and flame spread rate present the same chang trend that increase first and then decrease and finally reach a steady state, flame pulsation frequency increases first and then decreases since sample width more than 20cm. The heat transfer from flame and pyrolysis zone to virgin region is proportional to the height of flame, and mainly dominated by radioactive regime.

In this paper, forward and inverse position solution are analyzed for a novel 2UPR/1SPR special 3-DOF parallel mechanism. The moving platform of this mechanism have 1 translational DOF and 2 rotational DOFs. The inverse position solution of this mechanism was deduced by analytical method. The decoupling equation of position and posture parameters are formulated on the basis of structural constraint of the concerned mechanism. The forward position solution of this mechanism is achieved by numerical method.

A novel 3SPS/UP spatial 3-DOF parallel mechanism is studied. The studied mechanism is composed of a moving platform connected to a fixed platform by means of four actuated limbs. The three of the four limbs are SPS serial manipulators and the other one is UP serial manipulator. Each SPS limb consists of a spherical pair S connecting to the fixed platform, a prismatic pair P, and another spherical pair S connecting to the moving platform. The UP limb consists of eight Hoke joints U and a prismatic pair P. One side of the UP limb is connected to the fixed platform by Hoke joints U and another side is fixed to the moving platform. Moving character of this mechanism are analyzed by using the theory of screws. The moving platform of this mechanism have three translational DOFs