1. Extraction of Plant Activator Protein from Botrytis Strain and Activity Detection
- Author
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Yan Wei Cheng, Yi Li, Chao Jun He, Xi Ning Du, Hui Yuan Ya, Yan Zhao Zhang, Bing Yue Wu, Da Xia Ding, and Lin Tang
- Subjects
food.ingredient ,food ,Biochemistry ,Strain (chemistry) ,Germination ,Chemistry ,Activity detection ,Extraction (chemistry) ,food and beverages ,General Medicine ,Single band ,Mycelium ,Botrytis - Abstract
In this paper, an activator protein was isolated and identified from crude protein of Botrytis Spp which was separated from the soil of the wheat field with the method of mycelium recycling, breaking and centrifuging,etc. The activator protein appeared a single band on SDS-PAGE whose molecular weight was about 42kD. At the same time, it had a preliminary activity test and investigated on this activator protein. This study indicated that this activator protein is able to promote wheat germination after the deal with it, especially when the density is 3μg/mL, the seed germination is the best. So it is proved that the plant activator protein has really the biological function of improving the wheat germination.
- Published
- 2014
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