1. Three-dimensional microscopic imaging through scattering media based on in-line phase-shift digital holography
- Author
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Eriko Watanabe, Yutaka Kano, Kanami Ikeda, Manami Ohta, Yoko Miyamoto, Mitsuo Takeda, Shutaro Kodama, and Wolfgang Osten
- Subjects
Physics ,Scattering ,business.industry ,Image quality ,Phase (waves) ,Speckle noise ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,010309 optics ,Optics ,0103 physical sciences ,Line (geometry) ,Spatial frequency ,Electrical and Electronic Engineering ,business ,Engineering (miscellaneous) ,Image resolution ,Digital holography - Abstract
Microscopic three-dimensional imaging and phase quantification for objects hidden behind a scattering medium by using in-line phase-shift digital holography are proposed. A spatial resolution of 1.81 µm and highly accurate quantitative phase imaging are demonstrated for objects behind a scatter plate. Three-dimensional imaging was confirmed using objects with a depth difference of 1.32 mm. Further, imaging was performed using rat skin as a demonstration for imaging through a complex multilayer scattering medium, where a spatial resolution close to the theoretically predicted value was achieved by experiment.
- Published
- 2019
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