1. Supercontinuum source-based multi-contrast optical coherence tomography for rat retina imaging
- Author
-
Jia Pu Syu, Waradee Buddhakosai, Wen Chuan Kuo, Chang Chih Ke, Shih Hwa Chiou, and Shih Jen Chen
- Subjects
Point spread function ,Materials science ,medicine.diagnostic_test ,media_common.quotation_subject ,Fundus photography ,01 natural sciences ,Article ,Atomic and Molecular Physics, and Optics ,Supercontinuum ,010309 optics ,03 medical and health sciences ,0302 clinical medicine ,Optical coherence tomography ,In vivo ,0103 physical sciences ,030221 ophthalmology & optometry ,medicine ,Contrast (vision) ,sense organs ,Tomography ,Preclinical imaging ,Biotechnology ,Biomedical engineering ,media_common - Abstract
This study proposed an ultrahigh-resolution multi-contrast optical coherence tomography system integrated with fundus photography for in vivo retinal imaging of rodents. A supercontinuum light source was used in the system, providing an axial resolution of less than 3 µm within 1.8 mm (in the tissue). Three types of tissue contrast based on backscattered intensity, phase retardation, and microvasculature at a capillary level can be simultaneously obtained using the proposed system. Pigmented Long-Evans, non-pigmented (albino) Sprague Dawley, and Royal College of Surgeons rats were imaged and compared. In vivo imaging results were validated with histology.
- Published
- 2018