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Temperature and CO2 concentration affect the yield and quality of mushrooms. In particular, high CO2 concentration due to mushroom respiration induces specific physiological disorders of mushrooms. The objective of this study was to quantify the CO2 emission rate of King Oyster mushrooms (Pleurotus eryngii (DC.) Quel) as a function of temperature and growth stage during the cultivation including thinning processes. CO2 emission rates of the substrate including mycelium before and after thinning were significantly different. In the respiration model, the maintenance and CO2 production coefficients of fruit bodies were expressed as quadratic equations according to temperature. The total CO2 emission rate of a bottle of mushroom estimated by the model were validated with measured ones (R 2 = 0.71). The CO2 emission rates of the mushroom showed exponential and quadratical increases with growth stage and temperature at 16 to 25℃, respectively. The CO2 emission rate models developed for King Oyster mushroom can be utilized to control the CO2 concentration and temperature in mushroom cultivation facilities.

For developing a canopy photosynthesis model, an efficient method to measure the photosynthetic rate in a growth chamber is required. The objective of this study was to develop a method for establishing canopy photosynthetic rate curves of romaine lettuce (Lactuca sativa L.) with light intensity and CO2 concentration variables using controlled growth chamber. The plants were grown in plant factory modules, and the canopy photosynthesis rates were measured in sealed growth chambers made of acrylic (1.0 x 0.8 x 0.5 m). First, the canopy photosynthetic rates of the plants were measured, and then the time constants were compared between two application methods: 1) changing light intensity (340, 270, 200, and 130 μmol·m-2·s-1) at a fixed CO2 concentration (1,000 μmol·mol-1) and 2) changing CO2 concentration (600, 1,000, 1,400, and 1,800 μmol·mol-1) at a fixed light intensity (200 μmol·m-2·s-1). Second, the canopy photosynthetic rates were measured by changing the light intensity at a CO2 concentration of 1,000 μmol·mol-1 and compared with those measured by changing the CO2 concentration at a light intensity of 200 μmol·m-2·s-1. The time constant when changing the CO2 concentration at the fixed light intensity was 3.2 times longer, and the deviation in photosynthetic rate was larger than when changing the light intensity. The canopy photosynthetic rate was obtained stably with a time lag of one min when changing the light intensity, while a time lag of six min or longer was required when changing the CO2 concentration. Therefore, changing the light intensity at a fixed CO2 concentration is more appropriate for short-term measurement of canopy photosynthesis using a growth chamber.