1. Some Studies of the Effect of Thyroid Hormone on the RNA Synthesis of the Anterior Pituitary Gland of Rats
- Author
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O. N. Miller, K. L. Lee, and Cyril Y. Bowers
- Subjects
Male ,Thyroid Hormones ,medicine.medical_specialty ,Hydrocortisone ,Uracil Nucleotides ,medicine.medical_treatment ,Glycine ,In Vitro Techniques ,Tritium ,Endocrinology ,Anterior pituitary ,Internal medicine ,medicine ,Animals ,Testosterone ,Castration ,Uridine ,Triiodothyronine ,Chemistry ,Adrenalectomy ,Thyroid ,Thyroidectomy ,RNA ,Rats ,medicine.anatomical_structure ,Depression, Chemical ,Pituitary Gland ,Protein Biosynthesis ,Hormone - Abstract
The effect of thyroid activity on the rat anterior pituitary gland (APG) RNA synthesis was studied by measuring the incorporation of 3H-uridine into RNA. The incorporation of 3H-uridine both in vivo and in vitro into the RNA of APG was enhanced in thyroidectomized (THX) rats and depressed after administration of triiodothyronine (T3) to the THX animal. The thyroidectomy-enhanced in vitro uridine incorporation could be observed one week after the operation and the T3 inhibition on 3H-uridine incorporation was evident 4 hr after administration of 10 μg/100 g body weight of the hormone. Under the same experimental conditions, the effects of thyroidectomy and of T3 on liver RNA synthesis were the opposite of those found in the APG; thyroidectomy decreased and T3 increased the RNA synthesis in the liver. The incorporation of 3H-uridine into brain RNA was not altered under the different thyroid states. Adrenalectomy, castration or administration of hydrocortisone or testosterone to THX rats had no effect on 3H-...
- Published
- 1968