1. LARP-1 promotes oogenesis by repressingfem-3in theC. elegansgermline
- Author
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Esther Zanin, Anne Pacquelet, Rafal Ciosk, Monica Gotta, Claudia Scheckel, De Villemeur, Hervé, Institute of Biochemistry, Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Ludwig Institute for Cancer Research, University of California [San Diego] (UC San Diego), University of California (UC)-University of California (UC), Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Friedrich Miescher Institute for Biomedical Research (FMI), Novartis Research Foundation, Department of Genetic Medicine and Development, University of Geneva School of Medicine, University of California-University of California, Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)
- Subjects
Male ,Protein family ,MAP Kinase Signaling System ,Caenorhabditis elegans Proteins/genetics/*metabolism ,RNA-binding protein ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Biology ,Mitogen-Activated Protein Kinase 1/genetics/metabolism ,Spermatozoa/physiology ,Oogenesis ,Germline ,03 medical and health sciences ,0302 clinical medicine ,Sex Determination Processes/*physiology ,[SDV.BDD] Life Sciences [q-bio]/Development Biology ,Animals ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,ddc:612 ,[SDV.BC] Life Sciences [q-bio]/Cellular Biology ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,Transcription factor ,Phylogeny ,[SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology ,030304 developmental biology ,Mitogen-Activated Protein Kinase 1 ,Genetics ,0303 health sciences ,Oogenesis/genetics/*physiology ,RNA-Binding Proteins ,[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology ,Cell Biology ,Sex Determination Processes ,biology.organism_classification ,Spermatozoa ,Ubiquitin ligase ,Caenorhabditis elegans/genetics/metabolism/*physiology ,Mutation ,Oocytes ,biology.protein ,Female ,RNA-Binding Proteins/*metabolism ,Oocytes/physiology ,030217 neurology & neurosurgery ,Cullin - Abstract
International audience; LA-related protein 1 (LARP-1) belongs to an RNA-binding protein family containing a LA motif. Here, we identify LARP-1 as a regulator of sex determination. In C. elegans hermaphrodites, a complex regulatory network regulates the switch from sperm to oocyte production. We find that simultaneous depletion of larp-1 and the Nanos homologue nos-3 results in germline masculinization. This phenotype is accompanied by a strong reduction of the levels of TRA-1, a GLI-family transcription factor that promotes oogenesis. TRA-1 levels are regulated by CBC(FEM-1), a ubiquitin ligase consisting of the FEM proteins, FEM-1, FEM-2 and FEM-3 and the cullin CUL-2. We show that both the masculinization phenotype and the reduction of TRA-1 levels observed in nos-3;larp-1 mutants require fem-3 activity, suggesting that nos-3 and larp-1 regulate the sperm-oocyte switch by inhibiting the fem genes. Consistently, fem-3 mRNA levels are increased in larp-1 mutants. By contrast, levels of fem-3 mRNA are not affected in nos-3 mutants. Therefore, our data indicate that LARP-1 and NOS-3 promote oogenesis by regulating fem-3 expression through distinct mechanisms.
- Published
- 2010