1. Inflammation associated weight cycling contributes to an obesogenic memory phenotype in adipose tissue
- Author
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Sierra J McDonald, Alexander T Sougiannis, Brandon Vanderveen, Taryn Cranford, Reilly T Enos, Kandy Velazquez, Ioulia Chatzistamou, Daping Fan, and Angela E Murphy
- Subjects
Immunology ,Immunology and Allergy - Abstract
Energy expenditure through physical activity and dieting promotes weight loss. However, sustaining a healthy weight can be challenging and often a pattern known as weight cycling emerges. We evaluated the inflammatory status of mice undergoing weight cycling. Male C57BL/6 mice were randomized into four groups (n=10/group): low fat diet (LFD) for 32 weeks (LFD), high fat diet (HFD) for 32 weeks (HFD), LFD for 28 weeks then changed to HFD for 4 weeks (LFD->H), HFD for 21 weeks then changed to LFD for 7 weeks and then changed to HFD for 4 weeks (HFD->L->H). LFD->H and HFD->L->H mice did not significantly differ in body weight, body fat weight, or body fat percentage, but were significantly higher than LFD mice while remaining lower than HFD mice (pL->H mice had smaller adipocytes of the epididymal fat pad compared to HFD and LFD->H mice which both had larger adipocytes (pL->H mice compared to LFD and LFD->H mice. This was consistent with more M1-type macrophages (pL->H mice compared to LFD->H. Liver histopathological and protein analysis revealed HFD->L->H mice had greater NASH and NAS (p
- Published
- 2020