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Introduction: Prevention of accidents and work related diseases, are not allowed regardless of the safety of employees, customers, contractors and other persons. Assessment of individual safety management activities could reduce many losses. Present study aimed to evaluate the safety management of a household appliance manufacturing company. .Material and Method: This study has done in a household appliance manufacturing company in Damavand city. Two questionnaires were firstly designed based on the weighted scores. The questionnaire 1 consisted of 4 indicators: Safety of machinery, Electrical safety, Risk assessment and Fire safety. Questionnaire 2 consisted of 11 sub indicators. Both questionnaires were completed by 30 HSE experts and supervisors. Reliability of questionnaires was based on cronbachs alpha coefficient. the safety status of each unit was determined and scored using information acquired by the questionnaires. Lastly, the safety of the entire company was determined. .Result: Results showed that in safety management: the pressing and store house were in a good range of 66.66 and 60.12 points. Powder painting, enameling, laboratory were in a average range of 56.25, 55.92 and 54.15 points. Assembling and door storage were in a week range of 46.06 points. .Conclusion: The findings showed that the safety status in the studied appliances company is in average range with 55.45 points. Therefore, it is recommended that the safety indicators should be improved for the betterment of the safety management in the company.

Background and Objectives: In clinical diagnostics, molecular methods are used to detect Mycobacterium tuberculosis bacilli (MTB) and to distinguish them from non-tuberculous mycobacteria (NTM). They are also used to make the right treatment decision for the patient as soon as possible. The aim of this study was to establish a rapid and novel multiplex PCR (mPCR) assay for the detection and differentiation of MTB and NTM in a single tube., Materials and Methods: 100 sputum samples positive for acid-fast bacilli (AFB) were included in this study. Mycobacterial culture, biochemical tests, and antibiotic susceptibility testing were performed on samples. After alkaline decontamination, total DNA was extracted from the samples. A primer pair targeting the rpoB gene, encoding the beta-subunit of RNA polymerase, was used to detect MTB and NTM, amplifying a 235-bp fragment of MTB and a 136-bp sequence of NTM. A pair of primers targeting a 190-bp fragment of the IS6110 region of MTB was also used to confirm the results. The sensitivity and specificity of the mPCR assay were evaluated using DNA extracted from standard strains. The amplified products were then analyzed by conventional agarose gel electrophoresis., Results: Of 100 AFB smear-positive sputum samples, 92 MTB DNA, 7 NTM DNA, and one mixed-infection sample were identified in a single tube using mPCR assay. There was no correlation between the AFB degree of smear positivity and PCR results. Of seven NTM isolates, 6 (86%) were resistant to rifampin, isoniazid, and ethambutol, the three first-line anti-tuberculosis drugs., Conclusion: A single-tube mPCR assay based on the rpoB gene provides a rapid and reliable means of detecting and differentiating MTB and NTM in sputum specimens., (Copyright © 2023 The Authors. Published by Tehran University of Medical Sciences.)