1. Carboxyamido-Triazole Modulates Retinal Pigment Epithelial and Choroidal Endothelial Cell Attachment, Migration, Proliferation, and MMP-2 Secretion of Choroidal Endothelial Cells.
- Author
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Hoffmann, Stephan, He, Shikun, Jin, Man Lin, Masiero, Laura, Wiedemann, Peter, Ryan, Stephen J., and Kohn, Elise C.
- Subjects
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ENDOTHELIUM , *RETINOIDS , *CELLS , *APOPTOSIS , *CELL death , *RETINAL degeneration , *BLOOD plasma - Abstract
Purpose: To determine the effect of the calcium signaling modulating drug carboxyamido-triazole (CAI) on substeps of exudative age-related macular degeneration (AMD) in vitro. Materials and Methods: Zymography and ELISA determined the effect of CAI on MMP-2 production of choroidal endothelial cells (CECs) stimulated by bFGF and VEGF. The effects of CAI on attachment of retinal pigment endothelial (RPE) cells/CECs onto fibronectin, laminin, collagen IV, and migration toward fibronectin were investigated. Proliferation induced by serum and bFGF (10 μ g/ml) with and without CAI (0.1–10 μ M) was measured by cell counting and [3] H-uptake. Viability and apoptosis of the exposed cells was assessed by an MTT and an apoptosis assay. Results: CAI inhibited serum- and bFGF-induced proliferation, cell attachment onto fibronectin and collagen IV, but only CEC attachment onto laminin. Inhibition of MMP-2 production was observed (10 μ M CAI). CAI reduced the cellular viability by apoptosis induction. Conclusions: CAI inhibits substeps of exudative macular degeneration and may be of value for the treatment of the disease. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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