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Hyssop is a medicinal plant species with multidirectional properties; it is also used as a spice. In Poland, Hyssop is grown on plantations. Essential oil is a dominating group of biologically active substances. The aim of present study was to evaluate the chemical composition of essential oil from Hyssop grown in south-eastern Poland. The Hyssop herb was collected in two dates: at the beginning of July and at the end of August. Essential oil was achieved by means of steam distillation for 3 hours. Diverse weather conditions during the study had no effects on the content and chemical composition of essential oil. Oil from Hyssop contained 52 compounds, including 1 unidentified. Main components of Hyssop essential oil were: isopinocamphone (22.53-28.74%), pinocamphone (11.41-17.99%), β-pinene (6.69-12.01%), elemol (5.02-7.57%), germacrene D (3.14-6.98%), and bicyclogermacrene (2.55-4.36%). [ABSTRACT FROM PUBLISHER]

Volatile components of marjoram (Origanum majoranaL.) essential oil obtained from the air-dried ground and unground herb, collected at full flowering (1st harvest), and from regrowth stems (2nd harvest) was analysed by GC-MS and GC-FID. The studied plants belong to thetrans-sabinene hydrate chemotype, and the average percentage of this compound was 49.12 %. The harvest of the herb at full flowering stage (July) allows to obtain an oil containing morecis-sabinene hydrate, sabinene as well ascis- andtrans- sabinene hydrate acetates than that obtained from the second harvest in October, from which we obtain, in turn, moretrans-sabinene hydrate, linalyl acetate, andE-caryophyllene in the oil. These modifications may be important for the practical application of marjoram essential oil and herbal raw material itself. The grinding of marjoram raw material should be considered to be a treatment that enhances the fragrance of the spice. The separation of low-value stems allows obtaining an oil with a higher proportion of sabinene andcis-sabinene hydrate. [ABSTRACT FROM AUTHOR]