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Silver pomfret fish (Stromateidae) is a family of Perciformes order. One species of this family is Pampus argenteus known as silver pomfret and lives in different parts of the Persian Gulf and Gulf of Oman. Therefore, the genetic relationship between its populations is so important. In this study, eight samples of Pampus argenteus were collected from four locations involved: Emam Khomeyni, Bushehr and Bandar Abbas in Persian Gulf and Chabahar in Gulf of Oman. Cytochrome b gene and D-loop region of mitochondrial DNA on these samples are analyzed. These genes are carried out using PCR with specific mtDNA primers and sequenced. Then, the evolutionary history was inferred by using the maximum-likelihood method. Evolutionary analyses were conducted in MEGA7. According to the phylogenetic trees, with translocations in Persian Gulf to Gulf of Oman, similarity decreases slowly. Chabahar1 sample in phylogenetic tree of cytochrome b is phylogenetically near China Sea samples. The reason may be migration from ocean to gulf throughout the centuries and Chabahar is in Gulf of Oman and locationally near the Arabian Sea and Indian Ocean. In D-loop region, results are similar to cytochrome b excepting one sample (Emam Khomeyni1). D-Loop in samples shows modification more than cytochrome b for the reason absence of protein product. [ABSTRACT FROM AUTHOR]

Silver pomfret, Pampus argenteus is an economically important seafood species. The fishery resource of pomfret in Indian waters shows a dwindling catch since the last few years and the pomfrets caught were mostly undersized which calls for immediate attempts for management of resources. An accurate definition of population structure is important for management of this species. The genetic stock structure of P. argenteus distributed along Indian coast was identified using analysis of 842 bp of complete ATPase 6/8 genes of mitochondrial DNA. Altogether, 83 silver pomfret ( P. argenteus) collected from 4 locations along Indian coast (Gujarat, Kerala, Tamil Nadu and West Bengal) were sequenced. Twenty four haplotypes were identified among 83 individuals with haplotype diversity (0.87) and nucleotide diversity (0.0025). The significant pair-wise FST and AMOVA values, between samples from West Bengal (east coast) and other locations along the west coast (Gujarat and Kerala) indicated the occurrence of distinct population structure in silver pomfret along the coast. [ABSTRACT FROM AUTHOR]

In this study, we sequenced and annotated the complete mitochondrial genome ofPampus argenteus(Perciformes: Stromateidae). The mitogenome is 17,098 bp in length, which contains 13 protein-coding genes, 2 rRNA genes, 23 tRNA genes and 2 non-coding regions: origin of light-strand replication (OL) and control region (D-loop). The overall nucleotide base composition ofP. argenteusmtDNA is A 30.35%, C 25.55%, G 15.28% and T 28.82%, with an A + T content of 59.17%. Except forND6gene and eight tRNA genes, all other mitochondrial genes were encoded on the heavy strand. The mitochondrial genome ofP. argenteusmay be helpful to the studies on conservation genetics and stock evaluation ofP. argenteusresource, as well as molecular phylogeny and species identification of Stromateidae. [ABSTRACT FROM AUTHOR]

Long PCR and primer walking methods are employed for determining the complete mitochondrial genome sequence ofPampus argenteus. The mitogenome is a circular molecule of 16,544 bp in length including the structure of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 2 non-coding regions (L-strand replication origin and control region). Within the control region, we identify the termination-associated sequence domain (TAS), central conserved domain (CSB-F, CSB-E and CSB-D), and three conserved sequence blocks (CSB-1, CSB-2 and CSB-3). [ABSTRACT FROM AUTHOR]