Search

Mir-10b has been reported as a key regulator of metastasis in many human tumours. Moreover, it has also been regarded as a prognostic marker and therapeutic target of colorectal cancer (CRC). Whether miR-10b could affect the metastasis and proliferation of CRC is unclear. MiR-10b expression was detected by qPCR in human CRC tissues and cell line, Luciferase activity was employed for miR-10b binding to the 3'UTR of KLF4, Genes expression were examined by western blot, and mRNA by qPCR. PI and Annexin V staining were used to evaluate the cell cycle and apoptosis. Cell proliferation was detected with MTT, and cell migration and invasion were performed with Transwell assay. We found that miR-10b expression was up-regulated in metastatic CRC tissues and cell lines. Inhibition of miR-10b prevented cancer cell metastasis and growth by inducing cell-cycle arrest and apoptosis in vitro. Moreover, we found that KLF4 was a direct target of miR-10b. MiR-10b inhibitor led to the up-regulation of E-cadherin expression and the down-regulation of cyclin D1, which were partly abrogated after silencing KLF4. [ABSTRACT FROM AUTHOR]

This paper analyses the 60-year-long dynamics of triple helix relations in the development of cleaner power generation technologies of a large state-owned Chinese power equipment manufacturer. It opens the black box of the firm and provides a whole picture of the firm's triple helix relations and the changes in the process. It addresses two research questions: How did the triple helix relations of the case firm evolve over the different stages of technology development? What's the special role of government in the evolution of triple helix relations in China? A heterodox top-down 'coordinated' model of triple helix relations is identified. [ABSTRACT FROM AUTHOR]