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A new cycloartane-type triterpenoid, 3β-acetoxy-9β,19-cyclolanost-24E-ene-1α,16β-diol-27-O-β-d-glucopyranoside (1), together with three known ones (2–4) were isolated from the aerial parts of Astragalus hoantchy. The structure of compound 1 was elucidated by detailed spectroscopic analyses including 1D and 2D NMR, HRESIMS, and IR experiments. All of the four compounds were isolated from A. hoantchy for the first time. The antifungal activities of compounds 1–4 against a phytopathogenic fungus (Alternaria solani) were evaluated with the result that compound 3 exhibited a moderate activity with an IC50 value of 284 μg/mL. [ABSTRACT FROM AUTHOR]

The Hequ Tibetan MastiffCanis lupus familiaris(Carnivora: Canidae) is a primitive breed of large dogs native to the northeastern Qinghai-Tibetan Plateau of China. In this study, its complete mitochondrial genome sequence has been assembled and characterized using high-throughput Illumina sequencing technology. The circular genome is 16 730 bp in length, and possesses all genomic components as typically found in most other metazoan mitogenomes. The gene arrangement is identical to those of most other vertebrates. Except forND4Lwith GTG as its start codon, all the other PCGs are initiated with an ATR (ATA/ATG) codon. Three distinct stop codons are employed, i.e. AGA forCYTB, TAA forATP6, ATP8, COX1, COX2, ND1, ND4L, ND5andND6, and an incomplete stop codon T forCOX3, ND2, ND3andND4. The nucleotide composition is asymmetric (31.6% A, 25.5% C, 14.2% G and 28.7% T) with an overall A + T content of 60.3%. These data would contribute to our better understanding its evolutionary history. [ABSTRACT FROM AUTHOR]