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Cannabis sativa L. is an extremely variable species. Even within the same cultivar plants can significantly differ in the content and profile of cannabinoids. Therefore, the best method for production of uniform plants and standardized raw material is vegetative propagation using clones. The aim of this study was to determine the content of cannabidiolic acid (CBDA), cannabidiol (CBD), Δ9-tetrahydrocannabinolic acid (Δ9-THCA), Δ9-tetrahydrocannabinol (Δ9-THC), cannabichromene (CBC), cannabigerol (CBG), and cannabinol (CBN) in clonally propagated plants of industrial hemp. One hundred and thirty-nine plants representing 17 different hemp genotypes were regenerated in vitro, hardened, and grown in a vegetation hall until harvest. Single plants of each accession were analyzed using high-performance liquid chromatography with UV/diode-array detection (HPLC-DAD/UV). The results revealed significant variability in the total cannabinoid content (0.55–5.18% in dry weight) among tested genotypes and within the Epsilon 68 cultivar. The highest content of total CBD (4.410%) was recorded for EPS/40 genotype, while the level of total Δ9-THC was below the allowed threshold (0.3%). Therefore, we can conclude that some clonally propagated plants provided reproducible hemp material as a potential source of cannabidiol. The results of this study will be useful for breeding and early selection of hemp genotypes. [ABSTRACT FROM AUTHOR]

The aim of the study was exploration of the properties of wool derived from sheep reared in Poland. Twenty-nine breeds of sheep diverse from the type of farming, e.g. for meat, dual-purpose breeds for wool-milk, and versatile wool are bred in Poland, but only few of them have uniform wool. Because there is a lack of data determining quality and quantity of Polish wool, the wool-based textile industry in Poland uses mainly imported raw material. Wool quality has an impact on processing efficiency and determines possibility of its further use. In order to assess the wool quality from the local sheep breeds (Polish Merino, Olkuska, Żelaźnieńska, Polish Lowland Sheep), fiber parameters were evaluated: average fiber diameter, length, curvature, and comfort factor. The wool was subjected to microscopic analysis and color examination. Results of the study showed that wool from the above-mentioned sheep breeds varies greatly in terms of quality. The obtained data helps fill the information gap on Polish wool. [ABSTRACT FROM AUTHOR]