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In this work in vitro evaluation of the reactivation potency of the newly synthesized reactivators for acetylcholinesterase (AChE; EC 3.1.1.7) is described. Using this method, reactivation potency of 21 potential reactivators of AChE inhibited by the nerve agent sarin has been evaluated. We have confirmed the fact that currently the most promising AChE reactivator, HI-6, is the most effective reactivator of sarin-inhibited AChE. There are only three AChE reactivators—HI-6, TO033 and TO047—able to satisfactorily reactivate sarin-inhibited AChE at the concentration 10 -5 M, which is nontoxic for human use. On the other hand, there are 14 AChE reactivators, that are able to reactivate sarin-inhibited AChE at the concentration 10 -3 M. However, this concentration of reactivator is probably toxic for human use. [ABSTRACT FROM AUTHOR]

In this work, the two rapid reversed-phase high-performance liquid chromatographic (RP-HPLC) methods were developed for the determination of seven homologues of benzalkonium bromide in hospital disinfectants. The first one uses column Purospher RP- I 8e and mixture of methanol-acetic acid and lithium perchlorate-octanesuiphonic acid aqueous solution as a mobile phase; the second method is performed on Waters Spherisorb Cyano column, and mobile phase consists of acetonitrile-acetate buffer. Both techniques use UV detection at 263 nm. The time of analysis is shorter than 15 mm, and limits of detection are bellow 0.005 mg/mL. [ABSTRACT FROM AUTHOR]