Search

The present study aimed to estimate the total dietary antioxidant capacity (TDAC) taken in by the Brazilian population. For analysis, the 36 most consumed foods in Brazil were evaluated. The foods were prepared according to their usual form of consumption and submitted to in vitro digestion. The daily intake of phenolics and flavonoids was estimated to be 2.31 ± 0.12 g and 374.12 ± 18.17 mg, respectively. The TDAC, evaluated as the ferric-reducing antioxidant power and as the trolox equivalent antioxidant capacity, was 10.3 and 9.4 mmol/d, respectively. The beverages, especially coffee, followed by beans, rice and salt bread were the most important sources of antioxidants. The average intake of phenolics and flavonoids of the Brazilian diet was comparatively higher than that estimated for several other countries. However, the contribution of fruits and vegetables to the phenolic intake and TDAC was minimal (4-6%). [ABSTRACT FROM AUTHOR]

We present a sensitive method for simultaneous determination of bisphenol A (BPA), benzophenone-3 (BP-3), 4-tert-octylphenol (t-OP),ortho-phenylphenol (OPP), four parabens (methyl, ethyl, propyl, butyl parabens) and five chlorophenols (2,4-dichlorophenol (2,4-DCP), 2,5-dichlorophenol (2,5-DCP), 2,4,5-trichlorophenol (2,4,5-triclorophenol), 2,4,6-trichlorophenol (2,4,6-TCP), and triclosan (TCS)), in human urine by high-pressure liquid chromatography (HPLC) mass spectrometry (MS). Samples were processed using enzymatic deconjugation of glucuronides followed by solid phase extraction (SPE) on a C18 cartridge and the eluate was concentrated. Analytes were separated by reversed-phase HPLC and then detected by atmospheric pressure chemical ionisation (APCI) MS and quantified by isotope dilution method. We describe details for optimisation of each step of the procedure. The sample treatment steps are straightforward and not labour-intensive and, therefore, permit a high sample throughput with excellent prospects for automation. This method shows low inter-day variation, and detection limits for most of the compounds are below 1 ng/mL in 1 mL of urine. The method accuracy was also verified by the analysis of proficiency testing urine samples. [ABSTRACT FROM AUTHOR]

In this article the authors discuss a study conducted by them related to urinary phenol and t,t-muconic acid (ttMA) in one of them who was exposed to airborne benzene. It states that though the study shows phenol and ttMA in urine sample, the limitation is that only one person was evaluated. It mentions that results of this study show that evaluation of single spot urine samples may lead to false positive results in terms of exposure to benzene.