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Copy number variation (CNV) is a type of genomic structural variation, and the research on it has flourished in recent years. According to the high-throughput sequencing data, we found that the copy number variation region of the GAL3ST1 gene was correlated with the growth traits of bovine. It is significant that we study the CNV of GAL3ST1 gene and process the association analysis between results of Q-PCR and growth traits of Chinese cattle. In this research, SPSS software was used to detect the distribution of GAL3ST1 gene copy number in four cattle breeds and the correlation of growth traits was analyzed. Correlation analysis showed that GAL3ST1 CNV had positive effects on some growth traits of bovine (p < 0.05). In addition, the study detects the expression of GAL3ST1 gene in different tissues of Xia'nan cattles on mRNA level. The result showed that GAL3ST1 gene has different expression conditions in different tissues, results showed that the expression level was high in intestine and low in liver tissue. In a word, we speculated that the GAL3ST1 gene can be used as a molecular marker and this study confirmed that the CNV of it can provide theoretical basis for molecular breeding of cattle in China. [ABSTRACT FROM AUTHOR]

Copy number variations (CNVs) were similar to single nucleotide polymorphisms (SNPs) and insertion-deletion (InDel), regarded as genetic variations in many species. CNV is defined as the variable change of DNA segment length compared with the reference genome, including gains or losses from 50 bp to several mega bases. The functions of USP16 gene are diverse, such as regulating the cell cycle, DNA damage, histone H2A deubiquitination or mitotic nuclear division. To analyze the relationship between CNV of USP16 gene and milk traits in Chinese Holstein, we used qPCR to detect the individuals of Chinese Holstein (n = 180). The results showed that the effect of USP16 gene CNV on daily milk yield and fat percentage had significant difference (p < 0.05). The gain was the advantage type in daily milk yield and the loss was the advantage type in fat percentage. Therefore, CNV of USP16 gene is an important factor of milk traits in Chinese Holstein. Meanwhile, it may be used as a molecular marker for assisted selection of milk traits in Chinese Holstein, which provides a theoretical basis for the genetic improvement of cow breeds in China. [ABSTRACT FROM AUTHOR]

Revealing the dynamics of soil organic carbon (Corg) and nutrients at aggregate scales is essential for improving our understanding of soil Corg mitigation and nutrient restitution in the Cunninghamia lanceolata plantations. In this study, soil Corg, total nitrogen (Ntot), available phosphorus (Pava), and exchangeable cations (including calcium (Ca2+), magnesium (Mg2+), potassium (K+), and sodium (Na+)) received the analysis in aggregate fractions acquired from 0 to 10 cm and 10–20 cm depths in the three different stand types, namely mixed stands of Cunninghamia lanceolata and Michelia macclurei (stand A), Cunninghamia lanceolata and Mytilaria laosensis (stand B), and pure stand of Cunninghamia lanceolata (stand C). Soil aggregates were classified into macro-aggregates (>2 mm), meso-aggregates (2–0.25 mm), and micro-aggregates (<0.25 mm) by the dry-sieving process. The two mixed stands displayed higher level of soil aggregate stability than the pure stand. Moreover, micro-aggregates acted as the main fractions that carried soil Corg, Ntot, and Pava, and both micro-aggregates and macro-aggregates referred to the main fractions that carried exchangeable cations. As for the soil nutrient stocks, only the exchangeable K+ stock of the pure stand dominated among the Corg and nutrient stocks, in addition, the rest of the other nutrient stocks of the mixed forests (stands A and B) took an advantage over the pure stand. Moreover, the Corg and nutrient stocks in stands A and B were reflected in the macro-aggregates, differently, those of stand C were mainly reflected in the micro-aggregates. Thus, selecting suitable broadleaf tree species mixed with Cunninghamia lanceolata can alleviate the reduction of soil aggregate stability and the loss of soil nutrients, thus promoting soil resources to be sustainably utilized and protecting soil quality and health in southern Guangxi of China. [ABSTRACT FROM AUTHOR]

Sainfoin (Onobrychis viciifolia) is one of the dominant legume forages distributed in Northern China. In our study, we assembled and annotated the structure of the complete chloroplast genome of sainfoin. The length of the circular genome is 122,102 bp. It contains 115 genes, including 79 protein-coding genes (68.7%), 31 tRNA genes (26.96%) and 5 rRNA genes (4.35%). The GC content of the total chloroplast genome of sainfoin is 34.58%. We construct the phylogenetic relationships between the chloroplast genome of sainfoin and the other 16 species by the Maximum likelihood (ML), and found sainfoin is most closely related to Hedysarum petrovii and Hedysarum taipeicum. [ABSTRACT FROM AUTHOR]

Agropyron pectinatum is a perennial forage widely cultivated in China, and it belongs to the Gramineous family. In this study, we assembled the complete chloroplast genome of A. pectinatum. The whole chloroplast genome of A. pectinatum is 135,041 bp in length, comprising a pair of inverted repeat (IR) regions (20,821 bp) that are separated by a large single copy (LSC) region (80,632 bp) and a small single copy (SSC) region (12,767 bp). The chloroplast genome of A. pectinatum contains 133 genes, and 87 of them are protein-coding genes, 38 are tRNA, and eight are rRNA genes. The chloroplast genome of A. pectinatum could provide valuable information for varieties identification and evolution of the Agropyron Gaertn. [ABSTRACT FROM AUTHOR]