1. Cold atmospheric plasma increases IBRV titer in MDBK cells by orchestrating the host cell network.
- Author
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Miao, Yujie, Han, Peiyu, Hua, Dong, Zhou, Renwu, Guan, Zhengbing, Lv, Qing, and Dai, Xiaofeng
- Subjects
LOW temperature plasmas ,REACTIVE oxygen species ,VIRAL vaccines ,INTRACELLULAR calcium ,MEMBRANE potential ,VIRUS diseases - Abstract
Enhancing virus multiplication could assist in the rapid production of vaccines against viral diseases. Cold atmospheric plasma (CAP), a physical approach relying on reactive oxygen species to achieve the desirable cellular outcome, was shown to be effective in enhancing virus propagation, where bovine rhinotrachieitis virus and Madin-Darby Bovine Kidney cells were used as the modeling virus and cell line, respectively. CAP was shown to create synergies with virus infection in arresting host cells at the G2/M stage, decreasing cell membrane potential, increasing intracellular calcium level, and inducing selective autophagy. In addition, CAP was demonstrated to suppress virus-triggered immunogenic signaling as evaluated by IRF7 expression. We presented evidences on CAP-triggered maximization of host resources toward virus multiplication that is advantageous for viral vaccine production, and opened a novel regime for applying CAP in the sector of medical care and health. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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