1. SELDI-TOF serum proteomics and colorectal cancer: A current overview.
- Author
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Gemoll, Timo, Roblick, Uwe Johannes, Auer, Gert, Jörnvall, Hans, and Habermann, Jens Karsten
- Subjects
PROTEOMICS ,COLON cancer diagnosis ,MASS spectrometry ,BIOMARKERS ,LITERATURE reviews ,SERODIAGNOSIS ,BLOOD plasma - Abstract
Surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) is a widely used technology platform for diagnostic biomarker discovery in tissue, plasma and serum. High-throughput and simplicity of experimental procedures have allowed this technology to become an important research tool for biomarker discovery during the last years. This review provides an overview of SELDI-TOF functionality, its advantages and drawbacks and gives a current literature overview of colorectal cancer based serum biomarker detection. Further improvements in instrumentation sensitivity and labelling chemistries will enable detection of novel, tissue-leakage biomarkers in serum. However, major emphasis should be given on subsequent identification of differentially observed protein peaks detected by SELDI-TOF. Clinical validation in large patient cohorts will then allow transferring novel biomarkers into clinical use. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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