Search

This analysis evaluates the characteristics of conventional nanofluid heat transfer with those of emerging hybrid nanofluid. Hybrid nanofluid, a new type of conventional fluid, has been used toward the enhancement of heat transfer in the boundary layer flow. This research also prompts that 0.001 solid volume fraction of Gold (Au) is fixed, then consequently, various solid volume fractions of Molybdenum disulfide (Mo) are added into the mixture with water as the base fluid to form Au - Mo/water hybrid nanofluid. Using the necessary similarity variables, the governing non-linear partial differential equations are resolved into a system of coupled ordinary non-linear differential equations. The transformed equations are numerically solved by employing the Runge-Kutta Fehlberg method with shooting technique. The numerical parametric analysis, which has been conducted to explore the effects of different physical parameters involved in the problem, provides comparisons. The present problem is new, original, with many substantial findings in the current industry for practical problems. It is found that dual solutions exist up to a certain value of the decreases as nanoparticle volume fractions for Molybdenum disulfide increase. [ABSTRACT FROM AUTHOR]

Copyright of Gynecological Endocrinology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Purpose: To identify the disease-causing gene of a four-generation Chinese family with congenital cataract. Methods: To screen the disease-causing gene of the family, six disease genes of congenital cataract are screened by direct DNA sequencing, the cDNA of wild-type (WT) MIP gene, and P191R mutant MIP gene (MT) were constructed into pEGFP-C1 vector and pGH19 vector. The recombinant plasmids of pEGFP-C1, WT, and mutant MIP were transfected into Hela cell to check the localization and HEK293T cells to detect expression level of protein. The cRNA of WT and MT MIP gene were injected into Xenopus oocytes to measure the swelling rate. Results: A novel missense mutation c.572C>G（p.P191R）at exon 3 of the MIP gene was identified and co-segregated with disease in the Chinese family. The same amount of pEGFP-WT MIP and pEGFP- P191R MIP plasmids were transfected in Hela cells. Confocal microscopy imaging showed that WT MIP protein predominantly localized on the plasma membrane, the mutant protein was rich in the cytoplasm in Hela cells. Western blot results show that the expression level of P191R mutant MIP was significantly lower than WT MIPincell membrane enriched lysates in HEK293T cells. Xenopus oocytes swelling assay showed that the P191R mutation reduces the swelling rate of Xenopus oocytes. Conclusions: The novel missense mutation c.572C>G（p.P191R）at exon 3 of the MIP gene was identified in a Chinese family of congenital cataract. The mutation affects the traffic of MIP protein in the cells and reduces the expression level of MIP protein in the cell membrane. The mutation of MIP gene reduces the swelling ratio of Xenopus oocytes. [ABSTRACT FROM AUTHOR]

Mycophenolic acid (MPA) is a group of metabolite derived from several species of Penicillium, which shows potent bioactivity. In this study, a new derivative of MPA compound named penicacid D (1), was isolated from the marine derived fungus Penicillium sp. SCSIO sof101, along with seven known compounds (2-8). Their structures were elucidated based on the HR-ESI-MS and NMR data. Moreover, the 1H and 13C NMR data of compound 2 and the 13C NMR data of compound 3 are reported. Compounds 1, 4 and 6 exhibited weak activities against Escherichia coli (clinical isolation number 100385570) and Acinetobacter baumannii (clinical isolation number 100069). [ABSTRACT FROM AUTHOR]

A sensitive and selective liquid chromatography-mass spectrometry (LC-MS) method for determination of tolbutamide and its metabolite hydroxytolbutamide in rat plasma was developed and validated. The analytes and internal standard omeprazole were extracted from plasma by liquid-liquid extraction using ethyl acetate, and chromatographically separated on a Zorbax SB-C18 column (2.1 mm × 50 mm, 3.5 µm) using acetonitrile-0.1% formic acid as the mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode, and selected ion monitoring (SIM) mode used to quantify tolbutamide and its metabolite hydroxytolbutamide. The assay was linear over the range 10–20000 ng/mL for tolbutamide and 5–800 ng/mL for hydroxytolbutamide, with a lower limit of quantification (LLOQ) of 10 ng/mL for tolbutamide and 5 ng/mL for hydroxytolbutamide. Intra- and inter-day precision were less than 12% and the accuracy were in the range 88.8–109.7%. This developed method was successfully used for determination of tolbutamide and metabolite hydroxytolbutamide in rat plasma for pharmacokinetic study. [ABSTRACT FROM AUTHOR]

A sensitive and selective liquid chromatography-mass spectrometry (LC–MS) method for determination of sulfamethoxazole and trimethoprim in rat plasma was developed and validated. The analytes and internal standard (IS) were extracted from plasma by liquid–liquid extraction using ethyl acetate, and chromatography involved Agilent Extend-C18 column (2.1 mm x 50 mm, 3.5 um) using 0.1 formic acid in water, and acetonitrile with methanol (1:1, v/v) as a mobile phase with gradient elution. Detection involved positive ion mode electrospray ionization (ESI), and selective ion monitoring (SIM) mode was used for quantification of target fragment ions m/z 253.8 for sulfamethoxazole, m/z 290.9 for trimethoprim and m/z 294.9 for IS. The assay was linear over the range of 20–40000 ng/mL for sulfamethoxazole and 10–1000 ng/mL for trimethoprim, with a lower limit of quantitation (LLOQ) of 20 ng/mL for sulfamethoxazole and 10 ng/mL for trimethoprim. Intra- and inter-day precisions were less than 15% and the accuracies were in the range of 92.5%–109.3% for both sulfamethoxazole and trimethoprim. This developed method was successfully used for the determination of sulfamethoxazole and trimethoprim in rat plasma for pharmacokinetic study. [ABSTRACT FROM AUTHOR]