1. Biomolecular membrane protein crystallization.
- Author
-
Bolla, Jani Reddy, Su, Chih-Chia, and Yu, Edward W.
- Subjects
- *
CRYSTALLIZATION , *MEMBRANE proteins , *BIOMOLECULES , *GENOMES , *X-ray crystallography , *MOLECULAR structure , *LIPIDS - Abstract
Integral membrane proteins comprise approximately 30% of the sequenced genomes, and there is an immediate need for their high-resolution structural information. Currently, the most reliable approach to obtain these structures is X-ray crystallography. However, obtaining crystals of membrane proteins that diffract to high resolution appears to be quite challenging, and remains a major obstacle in structural determination. This brief review summarizes a variety of methodologies for use in crystallizing these membrane proteins. Hopefully, by introducing the available methods, techniques, and providing a general understanding of membrane proteins, a rational decision can be made about now to crystallize these complex materials. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF