Search

A simple single step ion exchange chromatography method has been developed for purification of SA from plant cell culture extracts. The optimum washing and elution solvents are 25 mM sodium phosphate (pH 7.0-7.5) and 0.3 M HCl in 60% acetonitrile, respectively. Using the developed method the SA recoveries were found 69-80% calculated from the whole purification process. In particular, in the second 10 mL elution, 70 µg SA in 1 g resin yielded 65% SA recovery, which provided a well-detectable amount of SA with a relatively low level of impurities as analyzed by 400 MHz 1H-NMR. [ABSTRACT FROM AUTHOR]