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Phytochemical research of Perilla frutescens aerial parts led to isolation of 12 secondary metabolites, including one new 3-benzoxepin glucoside, perillafrutoside A ( 1 ), one new megastigmane glycoside, perillafrutoside B ( 2 ), and 10 known compounds. Their chemical structures were identified based on 1D/2D NMR, HRESIMS, and ECD spectroscopic analyses. The structure of 2 was elucidated based on revision of the previously reported stereoisomer, (6 R ,9 R )-blumenyl α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside. Evaluation of their antimicrobial effect revealed that compounds 1 and 5-11 inhibit Enterococcus faecalis growth, compounds 6 , 7 and 9 suppress Staphylococcus aureus growth, whereas compounds 6 and 11 attenuate Candida albicans growth. This is the first report of the isolation of 3-5 , 8-10 and 12 from the genus Perilla and the antimicrobial effect of compounds 3 , 8 and 10 .

Using various chromatographic separations, two new cembranoids, ehrenbergols F and G ( 1 and 2 ), along with three known analogs ehrenbergol D ( 3 ), (+)-isosarcophine ( 4 ) and sinulariol Z 2 ( 5 ) were isolated from the soft coral Sarcophyton ehrenbergi . The structural elucidation was done by extensive analysis of the 1 D and 2 D NMR, HR-ESI-QTOF-MS as well as CD experiments. In addition, compounds 1 (IC 50 of 38.38 ± 2.89 µM), 3 (IC 50 of 37.14 ± 3.22 µM) and 4 (IC 50 of 45.01 ± 2.49 µM) revealed moderate inhibitory activity on LPS-induced NO production in RAW264.7 cells, whereas 2 (IC 50 of 73.32 ± 1.95 µM) and 5 (IC 50 of 64.48 ± 4.93 µM) exhibited weak effect.

From the MeOH extract of the Vietnamese sea cucumber Holothuria edulis , eight triterpene glycosides ( 1 - 8 ), including one new compound namely holothurin A 5 ( 1 ), were isolated by using various chromatographic separations. Their structures were established by spectroscopic experiments including 1D, 2D NMR and HR-ESI-MS. Holothurin A 5 ( 1 ) has a hydroperoxy group at C-25. To the best of our knowledge, this is the first report of this group in triterpene saponins obtained from sea cucumbers to date. In addition, the in vitro cytotoxicity against five human cancer cell lines (HepG2, KB, LNCaP, MCF7 and SK-Mel2) of all isolated compounds was also evaluated using SRB assays.

Ten briarane-type diterpenoids ( 1 - 10 ), including one new stereoisomer 17- epi -junceellolide B ( 1 ), were isolated from the MeOH extract of the Vietnamese gorgonian Junceella fragilis . Their structures were elucidated by spectroscopic experiments including 1D and 2D NMR, and HR-QTOF-MS. In addition, the in vitro cytotoxic activity against eight human cancer cell lines (LNCaP, HepG2, KB, MCF-7, SK-Mel2, HL-60, LU-1 and SW480) of all isolated compounds was evaluated by SRB assays.

Six pregnane steroids including one new compound namely 15β-hydroxypregna-4,20-dien-3-one (1), were isolated and structurally elucidated from the octocoral Carijoa riisei. The cytotoxic activity against a panel of eight human cancer cell lines of isolated compounds was also evaluated by SRB method. As the results, 18-acetoxypregna-1,4,20-trien-3-one (5) showed significant cytotoxicity against all the tested cell lines with the IC 50 values from 22.29 ± 1.47 to 48.73 ± 3.93 μM, whereas 15β-acetoxypregna-1,4,20-trien-3-one (3) and 20R-acetoxypregna-1,4-dien-3-one (6) only exhibited weak effect on KB cell line with IC 50 values of 93.62 ± 7.32 and 71.38 ± 5.45 μM, respectively.