1. Long intergenic noncoding RNA01134 accelerates hepatocellular carcinoma progression by sponging microRNA-4784 and downregulating structure specific recognition protein 1
- Author
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Ziming Liang, Qing Yang, Lizhen Dai, Shiyang Zheng, Yan Guo, and Shu-hong Yi
- Subjects
0301 basic medicine ,Carcinoma, Hepatocellular ,Blotting, Western ,Bioengineering ,Biology ,In Vitro Techniques ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Cell Line ,linc01134 ,03 medical and health sciences ,0302 clinical medicine ,ssrp1 ,Downregulation and upregulation ,microRNA ,medicine ,Humans ,Immunoprecipitation ,neoplasms ,Competing endogenous RNA ,Reverse Transcriptase Polymerase Chain Reaction ,Liver Neoplasms ,RNA ,General Medicine ,hepatocellular carcinoma ,medicine.disease ,Long non-coding RNA ,digestive system diseases ,mir‐4784 ,MicroRNAs ,030104 developmental biology ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Cancer research ,RNA, Long Noncoding ,long noncoding rna ,Structure specific recognition protein 1 ,Carcinogenesis ,TP248.13-248.65 ,Research Article ,Research Paper ,Biotechnology - Abstract
Dysregulation of long noncoding RNAs (lncRNAs) has been suggested to foster the carcinogenesis of hepatocellular carcinoma (HCC). To date, the role of long intergenic noncoding RNA01134 (LINC01134) in HCC have never been researched yet. Herein, we found that LINC01134 was highly expressed in HCC tissues in comparison with the matched normal liver tissues and increased LINC01134 expression correlated with shorter overall survival of patients with HCC. Additionally, we demonstrated LINC01134 downregulation significantly suppressed the proliferation ability and colony formation capacity of HCC cells. Furthermore, we revealed that LINC01134 functioned as a competitive endogenous RNA (ceRNA) for miR-4784 to upregulate structure-specific recognition protein 1 (SSRP1) in HCC cells. Meanwhile, miR-4784 inhibitor or restoration of SSRP1 could markedly attenuate the inhibitory effect of LINC01134 downregulation on HCC cells. Taken together, LINC01134 may promote the carcinogenesis of HCC at least partly via the miR-4784/SSRP1 axis. Therefore, LINC01134/miR-4784/SSRP1 axis should be developed as the promising therapeutic target for HCC., Graphical Abstract
- Published
- 2020