1. Canine parvovirus induces G1/S cell cycle arrest that involves EGFR Tyr1086 phosphorylation
- Author
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Xuanhao Zhang, Peiyu Han, Xiaofeng Dai, Yujie Miao, and Jianying Zhang
- Subjects
Microbiology (medical) ,Cell cycle checkpoint ,Parvovirus, Canine ,animal diseases ,viruses ,EGFR ,Immunology ,Infectious and parasitic diseases ,RC109-216 ,Biology ,Microbiology ,Cell Line ,Madin Darby Canine Kidney Cells ,Parvoviridae Infections ,03 medical and health sciences ,chemistry.chemical_compound ,Cyclin D1 ,Dogs ,Animals ,Epidermal growth factor receptor ,Virotherapy ,Canine parvovirus ,Phosphorylation ,030304 developmental biology ,EGFR inhibitors ,0303 health sciences ,030306 microbiology ,Tyrosine phosphorylation ,G1 Phase Cell Cycle Checkpoints ,ErbB Receptors ,Infectious Diseases ,chemistry ,cell cycle arrest ,Cancer cell ,Host-Pathogen Interactions ,S Phase Cell Cycle Checkpoints ,Cancer research ,biology.protein ,Cats ,Parasitology ,virotherapy ,Research Article ,Research Paper - Abstract
Canine parvovirus (CPV) has been used in cancer control as a drug delivery vehicle or anti-tumor reagent due to its multiple natural advantages. However, potential host cell cycle arrest induced by virus infection may impose a big challenge to CPV associated cancer control as it could prevent host cancer cells from undergoing cell lysis and foster them regain viability once the virotherapy was ceased. To explore CPV-induced cell cycle arrest and the underlying mechanism toward improved virotherapeutic design, we focus on epidermal growth factor receptor (EGFR), a cellular receptor interacting with TfR that mediates CPV-host interactions, and alterations on its tyrosine phosphorylation sites in response to CPV infection. We found that CPV could trigger host G1/S cell cycle arrest via the EGFR (Y1086)/p27 and EGFR (Y1068)/STAT3/cyclin D1 axes, and EGFR inhibitor could not reverse this process. Our results contribute to our understandings on the mechanism of CPV-induced host cellular response and can be used in the onco-therapeutic design utilizing CPV by preventing host cancer cells from entering cell cycle arrest.
- Published
- 2020