1. Rap1GAP impairs cell-matrix adhesion in the absence of effects on cell-cell adhesion.
- Author
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Vuchak LA, Tsygankova OM, and Meinkoth JL
- Subjects
- Adenoviridae genetics, Animals, Antigens, CD, Cadherins metabolism, Cell Adhesion, Cell Movement, Colonic Neoplasms genetics, Colonic Neoplasms pathology, Epithelial Cells metabolism, HCT116 Cells, Humans, RNA Interference, Rats, Rats, Wistar, Signal Transduction, Transfection, beta Catenin metabolism, rap GTP-Binding Proteins metabolism, Cell-Matrix Junctions metabolism, GTPase-Activating Proteins metabolism, Gene Expression Regulation, Neoplastic
- Abstract
The significance of the widespread downregulation of Rap1GAP in human tumors is unknown. In previous studies we demonstrated that silencing Rap1GAP expression in human colon cancer cells resulted in sustained increases in Rap activity, enhanced spreading on collagen and the weakening of cell-cell contacts. The latter finding was unexpected based on the role of Rap1 in strengthening cell-cell adhesion and reports that Rap1GAP impairs cell-cell adhesion. We now show that Rap1GAP is a more effective inhibitor of cell-matrix compared to cell-cell adhesion. Overexpression of Rap1GAP in human colon cancer cells impaired Rap2 activity and the ability of cells to spread and migrate on collagen IV. Under the same conditions, Rap1GAP had no effect on cell-cell adhesion. Overexpression of Rap1GAP did not enhance the dissociation of cell aggregates nor did it impair the accumulation of β-catenin and E-cadherin at cell-cell contacts. To further explore the role of Rap1GAP in the regulation of cell-cell adhesion, Rap1GAP was overexpressed in non-transformed thyroid epithelial cells. Although the formation of cell-cell contacts required Rap1, overexpression of Rap1GAP did not impair cell-cell adhesion. These data indicate that transient, modest expression of Rap1GAP is compatible with cell-cell adhesion and that the role of Rap1GAP in the regulation of cell-cell adhesion may be more complex than is currently appreciated.
- Published
- 2011
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