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Your search keyword '"STK39"' showing total 3 results
Start Over Descriptor "STK39" Publisher taylor & francis
3 results on '"STK39"'

1. Knockdown of long non-coding RNA CDKN2B-AS1 suppresses the progression of breast cancer by miR-122-5p/STK39 axis

Author

Mingliang Ning, Qingyuan Liu, Yanbai Wang, Qilun Liu, Shaojie Qin, and Xiaoyun Ding

Subjects
Breast Neoplasms, Bioengineering, Protein Serine-Threonine Kinases, Biology, Lncrna cdkn2b-as1, Applied Microbiology and Biotechnology, Mice, Breast cancer, In vivo, Cell Line, Tumor, miR-122-5p, MiR-122, medicine, Animals, Humans, Breast, skin and connective tissue diseases, stk39, human breast cancer, Gene knockdown, In vitro toxicology, Cancer, General Medicine, Middle Aged, medicine.disease, Epithelium, Long non-coding RNA, MicroRNAs, medicine.anatomical_structure, Gene Knockdown Techniques, Disease Progression, Cancer research, Female, RNA, Long Noncoding, TP248.13-248.65, Research Article, Research Paper, Biotechnology
Abstract

The lncRNAs have been made certain to take part in the development of most cancers in multiple ways. Here, our purpose is to making observation of the biological role and function of lncRNA CDKN2B-AS1 in human breast cancer. Twenty-eight pairs of breast cancer tissue and adjacent normal tissue from breast cancer patients were used to investigate the expression of CDKN2B-AS1 by qRT-PCR. And a lentivirus-shRNA guided CDKN2B-AS1 were to reduce its expression. The function of CDKN2B-AS1 was analyzed using a series of in vitro assays. Meanwhile, the xenograft model was used to further explicate the role of CDKN2B-AS1 in breast cancer. As for the results, there is a relative rich expression of CDKN2B-AS1 in breast cancer tissues compared with the corresponding adjacent normal tissues. Compared with the human breast epithelial cell line, the abundant expression of CDKN2B-AS1 in breast cancer cells were revealed as well. Then, knockdown CDKN2B-AS1 inhibited the malignant biological behaviors of MCF7 and T47D cells. In mechanism, CDKN2B-AS1 sponged the miR-122-5p to regulate STK39 expression. Furthermore, the inhibition effect with sh-CDKN2B-AS1 on breast cancer cells was alleviated by miR-122-5p inhibitor. Last, an in vivo model also confirmed that knockdown CDKN2B-AS1 retarded the growth of breast cancer. Our data concluded that knockdown of CDKN2B-AS1 suppresses the progression of breast cancer by miR-122-5p/STK39 axis.

Published
2021
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2. Knockdown of STK39 suppressed cell proliferation, migration, and invasion in hepatocellular carcinoma by repressing the phosphorylation of mitogen-activated protein kinase p38.

Author

Chen J, Zhou L, Yang J, Xie H, Liu L, and Li Y

Subjects
Animals, Cell Line, Tumor, Cell Movement genetics, Cell Proliferation genetics, Female, Gene Knockdown Techniques, Humans, Male, Mice, Mice, Nude, Middle Aged, Phosphorylation, p38 Mitogen-Activated Protein Kinases genetics, Carcinoma, Hepatocellular genetics, Carcinoma, Hepatocellular metabolism, Carcinoma, Hepatocellular pathology, Liver Neoplasms genetics, Liver Neoplasms metabolism, Liver Neoplasms pathology, Protein Serine-Threonine Kinases genetics, Protein Serine-Threonine Kinases metabolism, p38 Mitogen-Activated Protein Kinases metabolism
Abstract

Hepatocellular carcinoma (HCC) is a serious malignant tumor of the liver. It has been reported that serine/threonine kinase 39 (STK39) participates in tumorigenesis. However, the role of STK39 in HCC remains unknown. In this study, the qRT-PCR and western blot assay demonstrated that STK39 expression was enhanced in HCC patients and tissues. Moreover, CCK-8 and colony formation assays confirmed that knockdown of STK39 suppressed SK-HEP-1 and Huh7 cells proliferation. Furthermore, wound healing assay and transwell assay revealed that knockdown of STK39 repressed SK-HEP-1 and Huh7 cells migration and invasion. Interestingly, knockdown of STK39 reduced p-p38/p38 ratio and levels of c-Myc. Consistently, knockdown of STK39 inhibited the HCC tumor growth in vivo . In summary, knockdown of STK39 suppressed the proliferation, migration, and invasion of HCC cells by inducing the lower levels of p-p38, which might provide a novel therapeutic target for HCC.

Published
2021
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3. Knockdown of long non-coding RNA CDKN2B-AS1 suppresses the progression of breast cancer by miR-122-5p/STK39 axis.

Author

Qin S, Ning M, Liu Q, Ding X, Wang Y, and Liu Q

Subjects
Animals, Breast metabolism, Cell Line, Tumor, Disease Progression, Female, Gene Knockdown Techniques, Humans, Mice, MicroRNAs metabolism, Middle Aged, Protein Serine-Threonine Kinases metabolism, RNA, Long Noncoding metabolism, Breast Neoplasms genetics, Breast Neoplasms metabolism, Breast Neoplasms pathology, MicroRNAs genetics, Protein Serine-Threonine Kinases genetics, RNA, Long Noncoding genetics
Abstract

The lncRNAs have been made certain to take part in the development of most cancers in multiple ways. Here, our purpose is to making observation of the biological role and function of lncRNA CDKN2B-AS1 in human breast cancer. Twenty-eight pairs of breast cancer tissue and adjacent normal tissue from breast cancer patients were used to investigate the expression of CDKN2B-AS1 by qRT-PCR. And a lentivirus-shRNA guided CDKN2B-AS1 were to reduce its expression. The function of CDKN2B-AS1 was analyzed using a series of in vitro assays. Meanwhile, the xenograft model was used to further explicate the role of CDKN2B-AS1 in breast cancer. As for the results, there is a relative rich expression of CDKN2B-AS1 in breast cancer tissues compared with the corresponding adjacent normal tissues. Compared with the human breast epithelial cell line, the abundant expression of CDKN2B-AS1 in breast cancer cells were revealed as well. Then, knockdown CDKN2B-AS1 inhibited the malignant biological behaviors of MCF7 and T47D cells. In mechanism, CDKN2B-AS1 sponged the miR-122-5p to regulate STK39 expression. Furthermore, the inhibition effect with sh-CDKN2B-AS1 on breast cancer cells was alleviated by miR-122-5p inhibitor. Last, an i n vivo model also confirmed that knockdown CDKN2B-AS1 retarded the growth of breast cancer. Our data concluded that knockdown of CDKN2B-AS1 suppresses the progression of breast cancer by miR-122-5p/STK39 axis.

Published
2021
Full Text
View/download PDF

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