1. Genomic organization of the murine aminomethyltransferase gene (Amt).
- Author
-
Backofen B and Leeb T
- Subjects
- Amino Acid Sequence, Aminomethyltransferase, Animals, Base Composition, Base Sequence, Chromosome Mapping, DNA analysis, Genomic Library, Hyperglycinemia, Nonketotic enzymology, Hyperglycinemia, Nonketotic genetics, Introns, Mice, Molecular Sequence Data, Sequence Homology, Amino Acid, Hydroxymethyl and Formyl Transferases genetics
- Abstract
Aminomethyltransferase (Amt), also called glycine cleavage system T-protein is an important enzyme in glycine metabolism (EC 2.1.2.10). Mutations in this gene in humans lead to nonketotic hyperglycinemia, a fatal Mendelian disease. Here, we report the cloning and sequencing of the murine Amt gene. The murine Amt gene consists of nine closely spaced exons that are contained within approximately 5 kb of genomic DNA. It encodes a protein of 403 amino acids that is highly homologous to other mammalian aminomethyltransferases. The cis-acting promoter of the Amt gene is likely to be very short as immediately upstream of the murine Amt gene another gene termed Nicolin 1 gene (Nicn1) is located.
- Published
- 2002
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