1. Preparation of cryogel columns for depletion of hemoglobin from human blood
- Author
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Gözde Baydemir, Adil Denizli, Fatma Yılmaz, Nilay Bereli, Ali Derazshamshir, Sabire Yazıcı Fen Edebiyat Fakültesi, BAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümü, and Yılmaz, Fatma
- Subjects
Materials science ,Base (chemistry) ,Iminodiacetic acid ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,02 engineering and technology ,Methacrylate ,Hemolysis ,01 natural sciences ,Matrix (chemical analysis) ,Metal Chelate Affinity Sorbents ,Metal Chelate Sffinity Sorbents ,Hemoglobins ,chemistry.chemical_compound ,Hemoglobin Depletion ,Humans ,Chelation ,Epichlorohydrin ,Polyhydroxyethyl Methacrylate ,chemistry.chemical_classification ,Chromatography ,Imino Acids ,010401 analytical chemistry ,General Medicine ,021001 nanoscience & nanotechnology ,PHEMA Cryogel ,0104 chemical sciences ,chemistry ,Covalent bond ,IDA ,Hemoglobin ,0210 nano-technology ,Cryogels ,Biotechnology - Abstract
WOS: 000376136500005, PubMed: 26772759, In this study, we aimed to prepare the metal chelate affinity cryogels for the hemoglobin (Hb) depletion. Poly(2-hydroxyethyl methacrylate) (PHEMA) cryogels were selected as base matrix because of their blood compatibility, osmotic, chemical, and mechanical stability. Cryogels are also useful when working with the viscous samples such as blood, because of their interconnected macroporous structure. Iminodiacetic acid (IDA), the chelating agent, was covalently coupled with PHEMA cryogels after activation with the epichlorohydrin and then the Ni(II) ions were chelated to the IDA-bound cryogels. The depletion of the Hb from hemolysate was shown by SDS-PAGE.
- Published
- 2016