1. Surface metabolites of the brown alga Taonia atomaria have the ability to regulate epibiosis
- Author
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Jean-François Briand, Ahlem Othmani, Gérald Culioli, Mireille Ayé, Maëlle Molmeret, Laboratoire Electronique, Informatique et Image ( Le2i ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Matériaux Polymères Interfaces Environnement Marin - EA 4323 ( MAPIEM ), Université de Toulon ( UTLN ), Laboratoire Electronique, Informatique et Image [UMR6306] (Le2i), Université de Bourgogne (UB)-École Nationale Supérieure d'Arts et Métiers (ENSAM), Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM)-Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Laboratoire Matériaux Polymères Interfaces Environnement Marin - EA 4323 (MAPIEM), Université de Toulon (UTLN), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Arts et Métiers (ENSAM), and HESAM Université (HESAM)-HESAM Université (HESAM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement
- Subjects
0106 biological sciences ,0301 basic medicine ,Biocide ,Surface Properties ,Aquatic Science ,Phaeophyta ,01 natural sciences ,Applied Microbiology and Biotechnology ,Bacterial Adhesion ,Biofouling ,03 medical and health sciences ,Algae ,[ CHIM.ORGA ] Chemical Sciences/Organic chemistry ,Botany ,Mediterranean Sea ,ComputingMilieux_MISCELLANEOUS ,Water Science and Technology ,Chromatography ,biology ,Bacteria ,[CHIM.ORGA]Chemical Sciences/Organic chemistry ,010604 marine biology & hydrobiology ,Extraction (chemistry) ,Biofilm ,biology.organism_classification ,Seaweed ,Solvent ,030104 developmental biology ,Membrane ,Biofilms ,Metabolome ,Disinfectants - Abstract
This study aimed to improve understanding of the strategies developed by the Mediterranean seaweed Taonia atomaria to chemically control bacterial epibiosis. An experimental protocol was optimized to specifically extract algal surface-associated metabolites by a technique involving dipping in organic solvents whilst the integrity of algal cell membranes was assessed by fluorescent microscopy. This methodology was validated using mass spectrometry-based profiles of algal extracts and analysis of their principal components, which led to the selection of methanol as the extraction solvent with a maximum exposure time of 15 s. Six compounds (A–F) were identified in the resulting surface extracts. Two of these surface-associated compounds (B and C) showed selective anti-adhesion properties against reference bacterial strains isolated from artificial surfaces while remaining inactive against epibiotic bacteria of T. atomaria. Such specificity was not observed for commercial antifouling biocides and other molecules identified in the surface or whole-cell extracts of T. atomaria.
- Published
- 2016
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