1. New amperometric biosensors based on diamond paste for the assay of L- and D-pipecolic acids in serum samples.
- Author
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Stefan RI, Nejem RM, van Staden JF, and Aboul-Enein HY
- Subjects
- Biomarkers analysis, Biosensing Techniques instrumentation, Electrochemistry instrumentation, Electrodes, Humans, Infant, Newborn, Infant, Newborn, Diseases diagnosis, Liver Diseases diagnosis, Oils chemistry, Ointments chemistry, Paraffin, Pipecolic Acids blood, Sensitivity and Specificity, Stereoisomerism, Amino Acid Oxidoreductases chemistry, Biosensing Techniques methods, Diamond chemistry, Electrochemistry methods, Pipecolic Acids analysis, Serum chemistry
- Abstract
Monocrystalline natural diamond, L-amino acid oxidase (L-AAOD), D-amino acid oxidase (D-AAOD), and paraffin oil were used for the design of the modified diamond paste. The technique used for the direct voltammetric assay was differential pulse voltammetry (DPV) with applied potential pulse amplitude of 25 mV vs. Ag/AgCl. Using the new amperometric biosensors L-pipecolic acid (L-PA) and D-pipecolic acid (D-PA) were determined reliably from serum samples at 700 and 200 mV vs. Ag/AgCl, respectively, with low limits of detection.
- Published
- 2004
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