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5 results on '"F Clerici"'

1. Light chain deposition disease neuropathy resembling amyloid neuropathy in a multiple myeloma patient

Author

Gendarini A, Angelo Quattrini, Alfonso Mangoni, M Grassi, F Clerici, C Perin, M Borella, Raffaello Nemni, Grassi, M, Clerici, F, Perin, C, Borella, M, Gendarini, A, Quattrini, A, Nemni, R, and Mangoni, A

Subjects
Male, Pathology, medicine.medical_specialty, Biopsy, Fluorescent Antibody Technique, Dermatology, Light chain deposition disease, Polyneuropathies, Sural Nerve, medicine, Humans, Pure autonomic failure, Multiple myeloma, Aged, MED/26 - NEUROLOGIA, Nerve biopsy, medicine.diagnostic_test, business.industry, General Neuroscience, General Medicine, Amyloidosis, medicine.disease, IgG.lambda, Psychiatry and Mental health, Amyloid Neuropathy, Microscopy, Electron, Immunohistochemistry, Immunoglobulin Light Chains, Neurology (clinical), business, Multiple Myeloma, amyloidosis-light chain deposition disease-multiple myeloma-neuropathy, Polyneuropathy
Abstract

A 65-year-old man with IgG lambda multiple myeloma developed severe polyneuropathy with prominent thermal -pain sensory impairment and autonomic failure . although the clinical presentation suggested amyloid neuropathy, nerve biopsy showed the immunohistochemical and ultrastructural features typical of light chain deposition disease (LCDD). A precise morphologic and clinical description of LCDD neuropathy is given for the first time in the present report

Published
1998

2. Ex vivo thyroid fine needle aspirations as an alternative for MALDI-MSI proteomic investigation: intra-patient comparison.

Author

Piga I, Capitoli G, Clerici F, Mahajneh A, Brambilla V, Smith A, Leni D, L'Imperio V, Galimberti S, Pagni F, and Magni F

Subjects
Adult, Aged, Biopsy, Fine-Needle methods, Female, Humans, Male, Middle Aged, Proteomics methods, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods, Thyroid Gland pathology, Thyroid Neoplasms chemistry, Thyroid Neoplasms pathology, Thyroid Gland chemistry, Thyroid Neoplasms diagnosis
Abstract

Fine needle aspiration (FNA) is the reference standard for the diagnosis of thyroid nodules. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been successfully used to discriminate the proteomic profiles of benign and malignant thyroid FNAs within the scope of providing support to pathologists for the classification of morphologically borderline cases. However, real FNAs provide a limited amount of material due to sample collection restrictions. Ex vivo FNAs could represent a valuable alternative, increasing sample size and the power of statistical conclusions. In this study, we compared the real and ex vivo MALDI-MSI proteomic profiles, extracted from thyrocyte containing regions of interest, of 13 patients in order to verify their similarity. Statistical analysis demonstrated the mass spectra similarity of the proteomic profiles by performing intra-patient comparison, using statistical similarity systems. In conclusion, these results show that post-surgical FNAs represent a possible alternative source of material for MALDI-MSI proteomic investigations in instances where pre-surgical samples are unavailable or the number of cells is scarce.

Published
2021
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3. Expedient chemical synthesis of 75mer DNA binding domain of MafA: an insight on its binding to insulin enhancer.

Author

Pellegrino S, Annoni C, Contini A, Clerici F, and Gelmi ML

Subjects
Amino Acid Sequence, Binding Sites, Chromatography, High Pressure Liquid, Circular Dichroism, Humans, Insulin genetics, Microwaves, Molecular Sequence Data, Protein Binding, Protein Folding, Protein Structure, Tertiary, Solid-Phase Synthesis Techniques, Enhancer Elements, Genetic, Insulin metabolism, Leucine Zippers genetics, Maf Transcription Factors, Large chemical synthesis, Maf Transcription Factors, Large metabolism
Abstract

An expedient chemical synthesis of a 75mer peptide corresponding to the DNA binding domain (DBD, 227-301) of the human MafA leucine zipper transcription factor is reported. The application of microwave-assisted solid phase peptide synthesis (MW-SPPS) with a protocol modified respect to the standard one allowed obtaining the desired 75mer peptide in a short time with high quantity and optimal purity. MW-SPPS methodology was thus demonstrated as a valuable alternative to recombinant methods to obtain protein domains. Considering that recent findings suggest an involvement of MafA in the pathogenesis of diabetes mellitus, we also performed circular dichroism studies both on DBD folding and its interaction with MafA recognition element (MARE) on insulin enhancer. From our results, it was evicted that a disorder to order transition occurs after DBD interaction with insulin MARE which is mediated by specific structural elements on the N-terminus of the DBD.

Published
2012
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4. Cell-dependent kinase inhibitor 2A and 2B genetic variability in patients with Alzheimer's disease.

Author

Cortini F, Fenoglio C, Venturelli E, Villa C, Clerici F, Serpente M, Cantoni C, Fumagalli G, Mariani C, Bresolin N, Scarpini E, and Galimberti D

Subjects
Aged, Apolipoprotein E4 genetics, Confidence Intervals, DNA Mutational Analysis, Diagnostic Imaging methods, Female, Gene Frequency, Genotype, Humans, Linkage Disequilibrium, Male, Odds Ratio, Psychiatric Status Rating Scales, Alzheimer Disease genetics, Cyclin-Dependent Kinase Inhibitor p15 genetics, Cyclin-Dependent Kinase Inhibitor p16 genetics, Genetic Variation genetics
Published
2011
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5. CCL8/MCP-2 association analysis in patients with Alzheimer's disease and frontotemporal lobar degeneration.

Author

Villa C, Venturelli E, Fenoglio C, Clerici F, Marcone A, Benussi L, Ghidoni R, Gallone S, Cortini F, Scalabrini D, Serpente M, Binetti G, Cappa S, Mariani C, Rainero I, Bresolin N, Scarpini E, and Galimberti D

Subjects
Aged, Alzheimer Disease immunology, Alzheimer Disease physiopathology, Amino Acid Substitution genetics, DNA Mutational Analysis, Dementia immunology, Dementia physiopathology, Female, Gene Frequency genetics, Genetic Markers genetics, Genetic Testing, Genotype, Humans, Linkage Disequilibrium genetics, Male, Polymorphism, Single Nucleotide genetics, White People genetics, Alzheimer Disease genetics, Chemokine CCL8 genetics, Dementia genetics, Genetic Predisposition to Disease genetics
Abstract

CCL2/Monocyte Chemoattractant Protein (MCP)-1 and other chemokines sharing a similar sequence, including CCL8/MCP-2, are involved in neurodegeneration. A few Single Nucleotide Polymorphisms (SNPs) have been reported in CCL8/MCP-2, all of which are located in the same linkage block. One of them (rs1163763) leads to an aminoacidic substitution, implying a potential impact on the function of the protein. rs1133763 was tested for association in 219 patients with Alzheimer's disease (AD) and 209 with Frontotemporal Lobar Degeneration (FTLD) as compared with 231 age-matched controls. The distribution of CCL8/MCP-2 rs1133763 was not significantly different among patients with AD or FTLD and controls, even stratifying according to gender. CCL8/MCP rs1133763 SNP, or other variants in linkage disequilibrium with this variant, likely do not influence the susceptibility to AD or FTLD in Caucasians.

Published
2009
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