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Start Over Author "Assaad, F. F." Publisher springer-verlag
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2. Cauliflower mosaic virus P35S promoter activity in Escherichia coli.

Author

Assaad FF and Signer ER

Subjects
Base Sequence, Consensus Sequence, Escherichia coli drug effects, Escherichia coli enzymology, Kanamycin Kinase, Kanamycin Resistance genetics, Phosphotransferases metabolism, Restriction Mapping, Sequence Homology, Nucleic Acid, Transcription, Genetic, Escherichia coli genetics, Gene Expression Regulation, Enzymologic, Mosaic Viruses genetics, Phosphotransferases genetics, Promoter Regions, Genetic
Abstract

We present evidence that the cauliflower mosaic virus promoter P35S can direct expression of the bacterial neomycin phosphotransferase II (NPTII) gene in Escherichia coli. Transcription is initiated at several sites, the major one being located approximately 315 bases upstream of the plant start site. The nucleotide sequence directly preceding this start site is strongly homologous to the prokaryotic promoter consensus sequence. Thus constructs designed for introduction into plants can be expressed in E. coli.

Published
1990
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