1. Bilirubin oxidase from Magnaporthe oryzae: an attractive new enzyme for biotechnological applications
- Author
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Nicolas Mano, Fabien Durand, Christian H. Kjaergaard, Edward I. Solomon, Sébastien Gounel, Centre de recherches Paul Pascal (CRPP), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Department of Chemistry [Stanford], and Stanford University
- Subjects
Oxidoreductases Acting on CH-CH Group Donors ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,Oxygen reduction ,Bilirubin ,Gene Expression ,Biology ,010402 general chemistry ,01 natural sciences ,7. Clean energy ,Applied Microbiology and Biotechnology ,Remazol Brilliant Blue R ,Article ,Bilirubin oxidase ,Pichia pastoris ,Fungal Proteins ,03 medical and health sciences ,chemistry.chemical_compound ,Enzyme Stability ,Enzyme kinetics ,[INFO.INFO-BT]Computer Science [cs]/Biotechnology ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Verrucaria ,Magnaporthe oryzae ,General Medicine ,biology.organism_classification ,Biofuel cells ,Bilirubin detection ,0104 chemical sciences ,Kinetics ,Magnaporthe ,Enzyme ,chemistry ,Biochemistry ,Decolorization of dyes ,Myrothecium verrucaria ,Biotechnology - Abstract
10 pages; International audience; A novel bilirubin oxidase (BOD), from the rice blast fungus Magnaporthe oryzae, has been identified and isolated. The 64-kDa protein containing four coppers was successfully overexpressed in Pichia pastoris and purified to homogeneity in one step. Protein yield is more than 100 mg for 2 L culture, twice that of Myrothecium verrucaria. The kcat/Km ratio for conjugated bilirubin (1,513 mM−1 s−1) is higher than that obtained for the BOD from M. verrucaria expressed in native fungus (980 mM−1 s−1), with the lowest Km measured for any BOD highly desirable for detection of bilirubin in medical samples. In addition, this protein exhibits a half-life for deactivation >300 min at 37 °C, high stability at pH7, and high tolerance towards urea,making it an ideal candidate for the elaboration of biofuel cells, powering implantable medical devices. Finally, this new BOD is efficient in decolorizing textile dyes such as Remazol brilliant Blue R, making it useful for environmentally friendly industrial applications.
- Published
- 2012
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