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The provision of freshwater is essential for sustaining human life. Understanding the water provision modelling associated with the Land Use/Cover (LUC) change and climatic factors is vital for landscape water resource management. The Winike watershed is the largest tributary in the upper Omo Gibe basin of Ethiopia. This research aims to analyze the spatial and temporal change in the water yield to investigate the water yield contribution from the watershed based on the variation in input parameters. The Integrated Valuation of Ecosystem Services and Tradeoffs Tool (InVEST) water yield model was used to evaluate the spatial and temporal variation of the water yield in different years (1988, 1998, 2008 and 2018). The data required for this model include LUC data from satellite images, reference evapotranspiration, root depth, plant available water, precipitation, season factor (Z), and a biophysical table. The analysis of LUC change shows a rapid conversion of grazing land, shrubland, and forest land into cultivated land. There has been a significant variation in water provision, which increased from 1.83 × 10 9  m 3 in 1988 to 3.35 × 10 9  m 3 in 2018. Sub-watersheds 31, 32, and 39 in the eastern part of the watershed contributed more water due to higher precipitation and lower reference evapotranspiration. The major increase in the contribution of water yield was in built-up land by 207.4%, followed by bare land, 148.54%, and forest land by 63%. Precipitation had a greater impact on water yield estimation compared with the other input parameters. Hence, this research helps decision-makers to make informed decisions regarding new policies for LUC change improvement to maintain the water resources in the Winike watershed., (© 2021. The Author(s).)