1. A Fluorescent Probe for Diacetyl Detection
- Author
-
Axel Duerkop, Otto S. Wolfbeis, and Xiaohua Li
- Subjects
Time Factors ,Sociology and Political Science ,Cell medium ,Rhodamine B hydrazide ,Fluorescent probe - Rhodamine B hydrazide - Diacetyl - Cell medium ,Clinical Biochemistry ,Diacetyl ,Photochemistry ,Biochemistry ,chemistry.chemical_compound ,Fluorescent probe ,Rhodamine B ,Spectroscopy ,Fluorescent Dyes ,Original Paper ,Molecular Structure ,Rhodamines ,Hydrogen-Ion Concentration ,Fluorescence ,Clinical Psychology ,Fluorescence intensity ,Hydrazines ,Spectrometry, Fluorescence ,chemistry ,Solubility ,540 Chemie ,Luminescent Measurements ,Spectrophotometry, Ultraviolet ,Law ,Social Sciences (miscellaneous) ,Derivative (chemistry) - Abstract
A water-soluble fluorescent probe, rhodamine B hydrazide (RBH), was prepared and its properties for recognition of diacetyl were studied. The method employs the reaction of diacetyl with RBH, a colorless and non-fluorescent rhodamine B spiro form derivative to give a pink-colored fluorescent substance. In weakly acidic media, RBH reacts more selectively with diacetyl than with other carbonyls, causing a large increase in fluorescence intensity and thereby providing an easy assay for the determination of diacetyl.
- Published
- 2008