1. Stool Microbiota Composition Differs in Patients with Stomach, Colon, and Rectal Neoplasms
- Author
-
Milja Tikkanen, Pauli Puolakkainen, Monika Carpelan-Holmström, Sakari Knuutila, Tom Böhling, Homa Ehsan, Leo Lahti, Selja Koskensalo, Arto Kokkola, Hilpi Rautelin, Tiina Karla, Omar Youssef, Virinder Kaur Sarhadi, Medicum, Department of Pathology, University of Helsinki, Faculty of Medicine, II kirurgian klinikka, Clinicum, Department of Surgery, HUSLAB, Tom Böhling / Principal Investigator, Pauli Puolakkainen / Principal Investigator, HUS Abdominal Center, and Teachers' Academy
- Subjects
MECHANISM ,0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,Physiology ,Colorectal cancer ,Fecal microbiota ,Gastroenterology and Hepatology ,Disease ,Gut flora ,COLORECTAL-CANCER ,03 medical and health sciences ,Feces ,Human gut ,Stomach Neoplasms ,Internal medicine ,RNA, Ribosomal, 16S ,Gastroenterologi ,medicine ,Humans ,In patient ,skin and connective tissue diseases ,16S rRNA gene sequencing ,Aged ,Aged, 80 and over ,biology ,Rectal Neoplasms ,GUT MICROBIOTA ,Gastroenterology ,Hepatology ,Middle Aged ,medicine.disease ,biology.organism_classification ,Gastrointestinal neoplasms ,3. Good health ,Gastrointestinal Microbiome ,030104 developmental biology ,3121 General medicine, internal medicine and other clinical medicine ,CELLS ,Colonic Neoplasms ,Original Article ,Female ,sense organs ,Stomach colon ,Microbiota composition - Abstract
Background Microbial ecosystems that inhabit the human gut form central component of our physiology and metabolism, regulating and modulating both health and disease. Changes or disturbances in the composition and activity of this gut microbiota can result in altered immunity, inflammation, and even cancer. Aim To compare the composition and diversity of gut microbiota in stool samples from patient groups based on the site of neoplasm in the gastrointestinal tract (GIT) and to assess the possible contribution of the bacterial composition to tumorigenesis. Methods We studied gut microbiota by16S RNA gene sequencing from stool DNA of 83 patients, who were diagnosed with different GIT neoplasms, and 13 healthy individuals. Results As compared to healthy individuals, stools of patients with stomach neoplasms had elevated levels of Enterobacteriaceae, and those with rectal neoplasms had lower levels of Bifidobacteriaceae. Lower abundance of Lactobacillaceae was seen in patients with colon neoplasms. Abundance of Lactobacillaceae was higher in stools of GIT patients sampled after cancer treatment compared to samples collected before start of any treatment. In addition to site-specific differences, higher abundances of Ruminococcus, Subdoligranulum and lower abundances of Lachnoclostridium and Oscillibacter were observed in overall GIT neoplasms as compared to healthy controls Conclusion Our study demonstrates that the alterations in gut microbiota vary according to the site of GIT neoplasm. The observed lower abundance of two common families, Lactobacillaceae and Bifidobacteriaceae, and the increased abundance of Enterobacteriaceae could provide indicators of compromised gut health and potentially facilitate GIT disease monitoring. Electronic supplementary material The online version of this article (10.1007/s10620-018-5190-5) contains supplementary material, which is available to authorized users.
- Published
- 2018