1. Transcriptome analysis of fowl adenovirus serotype 4 infection in chickens
- Author
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Ruiai Chen, Ren Guangcai, Yuanyuan Yan, Fan Liu, Han Wang, and Miaorong Huang
- Subjects
Serotype ,Hydropericardium–hepatitis syndrome ,Adenoviridae Infections ,Notch signaling pathway ,Peroxisome proliferator-activated receptor ,RNA-Seq ,Biology ,Real-Time Polymerase Chain Reaction ,Serogroup ,Fowl adenovirus serotype 4 ,Pathogenesis ,Transcriptome ,03 medical and health sciences ,Virology ,Genetics ,Animals ,Molecular pathogenesis ,Receptor ,Molecular Biology ,Gene ,Poultry Diseases ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Original Paper ,030306 microbiology ,Sequence Analysis, RNA ,Aviadenovirus ,Gene Expression Profiling ,General Medicine ,chemistry ,Liver ,Host-Pathogen Interactions ,Differentially expressed genes ,RNA-seq ,Chickens - Abstract
Fowl adenovirus serotype 4 (FAdV-4) is a causative agent of inclusion body hepatitis and hydropericardium–hepatitis syndrome. These diseases cause considerable economic losses in the global poultry industry and are significant stressors for infected chickens. However, the molecular mechanisms of FAdV-4 pathogenesis are poorly understood. In the present study, we identified differentially expressed genes from the livers of FAdV-4-infected chickens using RNA-seq at 7, 14 and 21 days after FAdV-4 infection. We identified 2395 differentially expressed genes at the three time points. These genes were enriched in variety of biological processes and pathways including PPAR and Notch signaling, cytokine–cytokine receptor interactions and Toll-like receptor signaling pathways. The transcriptional data were validated by quantitative real-time PCR. Our results will assist in the understanding of the molecular pathogenesis of FAdV-4 infection and for developing novel antiviral therapies. Electronic supplementary material The online version of this article (10.1007/s11262-019-01676-w) contains supplementary material, which is available to authorized users.
- Published
- 2019