14 results on '"Young-Eun Park"'
Search Results
2. A high-fat diet has negative effects on tendon resident cells in an in vivo rat model
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Scott M. Bolam, Subhajit Konar, Young-Eun Park, Karen E. Callon, Josh Workman, A. Paul Monk, Brendan Coleman, Jillian Cornish, Mark H. Vickers, Jacob T. Munro, and David S. Musson
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Orthopedics and Sports Medicine ,Surgery - Abstract
Background Tendinopathy is a major complication of diet-induced obesity. However, the effects of a high-fat diet (HFD) on tendon have not been well characterised. We aimed to determine: [1] the impact of a HFD on tendon properties and gene expression; and [2] whether dietary transition to a control diet (CD) could restore normal tendon health. Methods Sprague–Dawley rats were randomised into three groups from weaning and fed either a: CD, HFD or HFD for 12 weeks and then CD thereafter (HF-CD). Biomechanical, histological and structural evaluation of the Achilles tendon was performed at 17 and 27 weeks of age. Tail tenocytes were isolated with growth rate and collagen production determined. Tenocytes and activated THP-1 cells were exposed to conditioned media (CM) of visceral adipose tissue explants, and gene expression was analysed. Results There were no differences in the biomechanical, histological or structural tendon properties between groups. However, tenocyte growth and collagen production were increased in the HFD group at 27 weeks. There was lower SOX-9 expression in the HFD and HF-CD groups at 17 weeks and higher expression of collagen-Iα1 and matrix metalloproteinase-13 in the HFD group at 27 weeks. THP-1 cells exposed to adipose tissue CM from animals fed a HFD or HF-CD had lower expression of Il-10 and higher expression of Il-1β. Conclusions In this rodent model, a HFD negatively altered tendon cell characteristics. Dietary intervention restored some gene expression changes; however, adipose tissue secretions from the HF-CD group promoted an increased inflammatory state in macrophages. These changes may predispose tendon to injury and adverse events later in life.
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- 2022
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3. Effect of Plant Container Type on Seed Potato (Solanum tuberosum L.) Growth and Yield in Substrate Culture
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Ju-Sung Im, Young-Eun Park, Hyun Jin Park, Chung-Gi Cheon, Jang-Gyu Choi, Jin-Hee Seo, Gyu-Bin Lee, Ji-Hong Cho, and Dong Chil Chang
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0106 biological sciences ,Plant growth ,Chemistry ,04 agricultural and veterinary sciences ,Solanum tuberosum ,Container (type theory) ,01 natural sciences ,Substrate (marine biology) ,Cutting ,Horticulture ,Yield (wine) ,Shoot ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Cultivar ,Agronomy and Crop Science ,010606 plant biology & botany ,Food Science - Abstract
The effects of container type on plant growth, yield and production cost of seed potatoes were investigated. Potato cultivars ‘Saebong’, ‘Geumsun’ (early-maturing) and ‘Haryeong’ (mid-maturing) were grown in four types of plant container: small (25 cm × 25 cm × 19 cm, L × W × H), medium (63 cm × 22 cm × 15 cm) and large (53 cm × 37 cm × 19 cm) plastic boxes and a styrofoam bed (100 cm × 52 cm × 31 cm). Shoot growth expressed as plant height was vigorous in the substrate culture system, especially in the styrofoam bed, whereas the roots tended to be longest in the small plastic box. ‘Haryeong’ showed the greatest shoot and tuber growth across plant container types. Tuber number (87.2–104.1 tubers m−2) and tuber weight (7.6–9 kg m−2) were greatest in the large plastic box and in the styrofoam bed. Furthermore, seed tuber (30–250 g) percentage of all tubers produced was high (93–95%), but no significant differences were observed among container types. The incidence of tuber physiological disorders was low (
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- 2021
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4. Analysis of genetic diversity and population structure among cultivated potato clones from Korea and global breeding programs
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Kwang Ryong, Jo, Seungho, Cho, Ji-Hong, Cho, Hyun-Jin, Park, Jang-Gyu, Choi, Young-Eun, Park, and Kwang-Soo, Cho
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Tetraploidy ,Plant Breeding ,Multidisciplinary ,Genotype ,Genetic Variation ,Polymorphism, Single Nucleotide ,United States ,Clone Cells ,Solanum tuberosum - Abstract
Characterizing the genetic diversity and population structure of breeding materials is essential for breeding to improve crop plants. The potato is an important non-cereal food crop worldwide, but breeding potatoes remains challenging owing to their auto-tetraploidy and highly heterozygous genome. We evaluated the genetic structure of a 110-line Korean potato germplasm using the SolCAP 8303 single nucleotide polymorphism (SNP) Infinium array and compared it with potato clones from other countries to understand the genetic landscape of cultivated potatoes. Following the tetraploid model, we conducted population structure analysis, revealing three subpopulations represented by two Korean potato groups and one separate foreign potato group within 110 lines. When analyzing 393 global potato clones, country/region-specific genetic patterns were revealed. The Korean potato clones exhibited higher heterozygosity than those from Japan, the United States, and other potato landraces. We also employed integrated extended haplotype homozygosity (iHS) and cross-population extended haplotype homozygosity (XP-EHH) to identify selection signatures spanning candidate genes associated with biotic and abiotic stress tolerance. Based on the informativeness of SNPs for dosage genotyping calls, 10 highly informative SNPs discriminating all 393 potatoes were identified. Our results could help understanding a potato breeding history that reflects regional adaptations and distinct market demands.
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- 2022
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5. Bone-Bound Bisphosphonates Inhibit Proliferation of Breast Cancer Cells
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Dorit Naot, Ian R. Reid, Usha Bava, Jillian Cornish, Jian-Ming Lin, and Young-Eun Park
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0301 basic medicine ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Cell ,Breast Neoplasms ,030209 endocrinology & metabolism ,Zoledronic Acid ,Bone and Bones ,03 medical and health sciences ,Prostate cancer ,0302 clinical medicine ,Endocrinology ,Breast cancer ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Orthopedics and Sports Medicine ,Cell Proliferation ,Bone Density Conservation Agents ,Diphosphonates ,Chemistry ,Cell growth ,Cancer ,Bisphosphonate ,medicine.disease ,medicine.anatomical_structure ,Zoledronic acid ,Apoptosis ,Cancer research ,Cattle ,030101 anatomy & morphology ,medicine.drug - Abstract
Bisphosphonates are used in treating patients with breast cancer. In vitro studies have shown that bisphosphonates act directly on tumour cells, inhibiting cell proliferation and inducing apoptosis. In most such studies, drugs were added to culture media exposing cells to high bisphosphonate concentrations in solution. However, since bisphosphonates bind to bone hydroxyapatite with high affinity and remain bound for very long periods of time, these experimental systems are not an optimal model for the action of the drugs in vivo. The aim of this study was to determine whether bone-bound zoledronate has direct effects on adjacent breast cancer cells. Bone slices were pre-incubated with bisphosphonate solutions, washed, and seeded with cells of the breast cancer cell lines, MCF7 or MDA-MB-231. Proliferation was assessed by cell counts and thymidine incorporation for up to 72 h. Inhibition of the mevalonate pathway was tested by measuring the levels of unprenylated Rap1A, and apoptosis was examined by the presence of cleaved caspase-8 on western blots. The proliferation rate of breast cancer cells on zoledronate-treated bone was significantly lower compared to cells on control bone. Other bisphosphonates showed a similar inhibitory effect, with an order of potency similar to their clinical potencies. Unprenylated Rap1A accumulated in MCF7 cells on zoledronate-treated bone, suggesting zoledronate acted through the inhibition of the mevalonate pathway. Accumulation of cleaved caspase-8 in MDA-MB-231 cells on bisphosphonate-treated bone indicated increased apoptosis in the cells. In conclusion, bone-bound zoledronate inhibits breast cancer cell proliferation, an activity that may contribute to its clinical anti-tumour effects.
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- 2019
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6. Differential regional infarction, neuronal loss and gliosis in the gerbil cerebral hemisphere following 30 min of unilateral common carotid artery occlusion
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Joon Ha Park, Hyun-Jung Kim, Minah Song, Young-Eun Park, In Koo Hwang, Young-Myeong Kim, Moo Ho Won, Ji Hyeon Ahn, Jun Hwi Cho, Tae-Kyeong Lee, Jae-Chul Lee, and Cheolwoo Park
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Brain Infarction ,Male ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Carotid Artery, Common ,Thalamus ,Hippocampus ,Gerbil ,Biochemistry ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,symbols.namesake ,0302 clinical medicine ,Glial Fibrillary Acidic Protein ,medicine ,Animals ,Carotid Stenosis ,Gliosis ,Neurons ,Cell Death ,biology ,business.industry ,Calcium-Binding Proteins ,Microfilament Proteins ,DNA-Binding Proteins ,030104 developmental biology ,medicine.anatomical_structure ,nervous system ,Cerebral cortex ,Astrocytes ,Cerebral hemisphere ,biology.protein ,Nissl body ,symbols ,Microglia ,Neurology (clinical) ,NeuN ,medicine.symptom ,Gerbillinae ,business ,030217 neurology & neurosurgery - Abstract
The degree of transient ischemic damage in the cerebral hemisphere is different according to duration of transient ischemia and cerebral regions. Mongolian gerbils show various lesions in the hemisphere after transient unilateral occlusion of the common carotid artery (UOCCA) because they have different types of patterns of anterior and posterior communicating arteries. We examined differential regional damage in the ipsilateral hemisphere of the gerbil after 30 min of UOCCA by using 2,3,5-triphenyltetrazolium chloride (TTC) staining, cresyl violet (CV) Nissl staining, Fluoro-Jade B (F-J B) fluorescence staining, and NeuN immunohistochemistry 5 days after UOCCA. In addition, regional differences in reactions of astrocytes and microglia were examined using GFAP and Iba-1 immunohistochemistry. After right UOCCA, neurological signs were assessed to define ischemic symptomatic animals. Moderate symptomatic gerbils showed several infarcts, while mild symptomatic gerbils showed selective neuronal death/loss in the primary motor and sensory cortex, striatum, thalamus, and hippocampus 5 days after UOCCA. In the areas, morphologically changed GFAP immunoreactive astrocytes and Iba-1 immunoreactive microglia were found, and their numbers were increased or decreased according to the damaged areas. In brief, our results demonstrate that 30 min of UOCCA in gerbils produced infarcts or selective neuronal death depending on ischemic severity in the ipsilateral cerebral cortex, striatum, thalamus and hippocampus, showing that astrocytes and microglia were differently reacted 5 days after UOCCA. Taken together, a gerbil model of 30 min of UOCCA can be used to study mechanisms of infarction and/or regional selective neuronal death/loss as well as neurological dysfunction following UOCCA.
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- 2018
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7. Characterization of trichome morphology and aphid resistance in cultivated and wild species of potato
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In-Taek Hwang, Young-Eun Park, Kwang-Soo Cho, Jin-Ho Kang, Min Kwon, Ju-Sung Im, Su-Young Hong, and Ji-Hong Cho
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0106 biological sciences ,0301 basic medicine ,Abiotic component ,Aphid ,Spots ,biology ,Macrosiphum euphorbiae ,fungi ,food and beverages ,Plant Science ,Horticulture ,biology.organism_classification ,Solanum tuberosum ,medicine.disease_cause ,01 natural sciences ,Trichome ,03 medical and health sciences ,030104 developmental biology ,Botany ,Infestation ,medicine ,Dew ,010606 plant biology & botany ,Biotechnology - Abstract
Trichomes are specialized epidermal structures that protect plants from abiotic and biotic stresses. Cultivated potato (Solanum tuberosum) is known to have both glandular and non-glandular trichomes. However, the criteria for trichome type classification have not been studied in detail. In addition, there is a poor understanding of the types of trichomes that exist in wild potato species. Here, the morphology and density of trichomes were compared between a representative cultivated potato variety and 17 wild potato species using Cryo Scanning Electron Microscopy (CryoSEM). Based on trichome morphology, the cultivated variety and each of the wild species were seen to have two glandular and two non-glandular trichome types. We classified the eighteen potato species into four groups using trichome type and density criteria. Groups I and II represented species with a higher density of glandular or non-glandular trichomes, respectively, on both abaxial and adaxial leaf surfaces. Group III represented species with a higher density of non-glandular trichomes on abaxial leaf surfaces alone. Group IV represented species with an overall lower trichome density on both abaxial and adaxial leaf surfaces, but which was formed of both glandular and non-glandular types. Honey dew spots were quantified following infestation with the aphid Macrosiphum euphorbiae to test whether trichome composition is associated with resistance to aphid feeding. Fewer honey dew spots were observed in the Group I representative species S. berthaultii and S. hougasii compared to that in species from other groups. Furthermore, correlation coefficient analysis showed that honey dew spot number was negatively associated with glandular trichome density. These results imply that glandular trichomes play an important role in aphid resistance and thus can be used for developing insect-resistant potato.
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- 2017
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8. Compositional analyses of diverse phytochemicals and polar metabolites from different-colored potato (Solanum tubersum L.) tubers
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Soon Ki Park, Soo-Yun Park, Yunsoo Yeo, Kwang-Soo Cho, Hyun Suk Cho, Seon-Woo Oh, Si Myung Lee, Young-Eun Park, and Wonhui Lee
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0106 biological sciences ,0301 basic medicine ,chemistry.chemical_classification ,Lutein ,Metabolite ,fungi ,food and beverages ,Primary metabolite ,Biology ,biology.organism_classification ,01 natural sciences ,Applied Microbiology and Biotechnology ,Amino acid ,03 medical and health sciences ,chemistry.chemical_compound ,Metabolic pathway ,030104 developmental biology ,chemistry ,Botany ,Solanum ,Sugar ,Carotenoid ,010606 plant biology & botany ,Food Science ,Biotechnology - Abstract
Lipophilic bioactive compounds and hydrophilic primary metabolites from potato (solanum tubersum L.) tubers with different-colored flesh (white-, yellow-, red-, and purple) were characterized. The carotenoid content was relatively higher in red-colored potatoes, in which lutein was most plentiful. Among the other lipophilic compounds analyzed, including policosanols, tocopherols, and phytosterols, octacosanol was measured in the largest amount, followed by β-sitosterol, irrespective of color variations. Forty-three hydrophilics consisting of amino acids, organic acids, sugars, and sugar alcohols and 18 lipophilics were subjected to data-mining processes. The results of multivariate statistical analyses clearly distincted the different varieties and separated red-fleshed potatoes from other color-fleshed potatoes according to abundance of amino acids, sugars, and carotenoids. This study confirmed the metabolic association-related biochemical pathway between metabolite characteristic and color differences in potato tubers. These results can facilitate understanding the metabolic differences among diverse colored potatoes and provide fruitful information for genetic engineering of potato cultivars.
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- 2017
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9. RbAp48 expression and neuronal damage in the gerbil hippocampus following 5 min of transient ischemia
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Hyang-Ah Lee, Ji Hyeon Ahn, Cheolwoo Park, Moo-Ho Won, Bora Kim, Dae Won Kim, Joon Ha Park, Young-Eun Park, Tae-Kyeong Lee, and Jae-Chul Lee
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0301 basic medicine ,medicine.medical_specialty ,Ischemia ,Hippocampus ,Hippocampal formation ,Astrocytes activation, delayed neuronal death ,Gerbil ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,lcsh:QH301-705.5 ,lcsh:R5-920 ,biology ,RbAp48 ,Chemistry ,Research ,food and beverages ,medicine.disease ,030104 developmental biology ,Histone ,Endocrinology ,medicine.anatomical_structure ,lcsh:Biology (General) ,nervous system ,Transient ischemia ,biology.protein ,Immunohistochemistry ,lcsh:Medicine (General) ,030217 neurology & neurosurgery ,Astrocyte - Abstract
Histone-binding protein RbAp48 has been known to be involved in histone acetylation, and epigenetic alterations of histone modifications are closely associated with the pathogenesis of ischemic reperfusion injury. In the current study, we investigated chronological change of RbAp48 expression in the hippocampus following 5 min of transient ischemia in gerbils. RbAp48 expression was examined 1, 2, 5, and 10 days after transient ischemia using immunohistochemistry. In sham operated gerbils, RbAp48 immunoreactivity was strong in pyramidal and non-pyramidal cells in the hippocampus. After transient ischemia, RbAp48 immunoreactivity was changed in the cornu ammonis 1 subfield (CA1), not in CA2/3. RbAp48 immunoreactivity in CA1 pyramidal neurons was gradually decreased and not detected at 5 and 10 days after ischemia. RbAp48 immunoreactivity in non-pyramidal cells was maintained until 2 days post-ischemia and significantly increased from 5 days post-ischemia. Double immunohistofluorescence staining revealed that RbAp48 immunoreactive non-pyramidal cells were astrocytes. At 5 days post-ischemia, death of pyramidal neurons occurred only in the CA1. These results showed that RbAp48 immunoreactivity was distinctively altered in pyramidal neurons and astrocytes in the hippocampal CA1 following 5 mins of transient ischemia. Ischemia-induced change in RbAp48 expression may be closely associated with neuronal death and astrocyte activation following 5 min of transient ischemia.
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- 2019
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10. Vitamin D status of patients with early inflammatory arthritis
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Young-Eun Park, Bo-Hyun Kim, Eun Kyung Park, Ji-Heh Park, Seung-Geun Lee, Geun-Tae Kim, and Sun Hee Lee
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Adult ,Male ,medicine.medical_specialty ,Gastroenterology ,vitamin D deficiency ,Rheumatology ,Internal medicine ,medicine ,Vitamin D and neurology ,Humans ,Vitamin D ,Aged ,Retrospective Studies ,business.industry ,Arthritis ,Radioimmunoassay ,Retrospective cohort study ,General Medicine ,Early Inflammatory Arthritis ,Middle Aged ,Vitamin D Deficiency ,medicine.disease ,Rheumatoid arthritis ,Immunology ,Female ,Seasons ,business ,Rheumatism - Abstract
The present study aimed to investigate the vitamin D status in patients with early inflammatory arthritis (EIA). We conducted a retrospective study among patients who presented with EIA at the outpatient rheumatology clinic of a tertiary referral center between March 2012 and February 2013. In total, 101 subjects with EIA (≥1 swollen joint and symptom duration of ≤6 months, not explained by another disease) and 101 healthy controls matched for age, sex, and the month of serum vitamin D measurements were enrolled. Serum 25-hydroxy vitamin D (25-OHD) concentrations were assessed by radioimmunoassay. Vitamin D “deficiency” and “severe deficiency” were defined as serum 25-OHD levels
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- 2014
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11. Vitamin C content of potato clones from Korean breeding lines and compositional changes during growth and after storage
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Kwang-Soo Cho, Hyun-Jun Kim, Hong-Sik Won, Young-Eun Park, Su-Young Hong, Hee-Jin Jeong, and Ji-Hong Cho
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Vitamin ,Antioxidant ,Vitamin C ,medicine.medical_treatment ,food and beverages ,Sowing ,Plant physiology ,Plant Science ,Horticulture ,Biology ,Ascorbic acid ,chemistry.chemical_compound ,chemistry ,Botany ,medicine ,Dormancy ,Cultivar ,Biotechnology - Abstract
Vitamin C is the main vitamin source in potato tubers, and its antioxidant activity is known to benefit human health. To improve the vitamin C content and understand its accumulation patterns, we selected 10 advanced clones with high vitamin C content from 268 clones. The levels of the selected clones ranged from 32.3 to 38.9 mg·100 g−1 fresh weight. Among them, four double-cropping clones with short dormancy were selected for further analysis. The vitamin C content of the potato tubers from six potato clones, including two control cultivars, ‘Dejima’ and ‘Chubaek’, was determined on specific days after planting (DAP) and during development. Developmental stages were categorized by weight: I (5–10 g), II (30–40 g), III (70–80 g), and IV (> 120 g). Vitamin C content was highest 90 DAP and dropped slowly by 100 and 110 DAP. Until 90 DAP, we found that developmental stages I and II showed higher contents of vitamin C; however, there were small differences by developmental stage (I to IV) by 100 and 110 DAP. In conclusion, vitamin C content was higher in younger tubers than in mature tubers, and its content was highest 90 DAP in the selected clones. During storage, vitamin C content significantly declined in all clones. The decrease was highest in H06035-4 and H06085-2 compared to ‘Chubaek’ and ‘Dejima’, and the differences between selected clones and control varieties narrowed after 4 months of storage. When we consider the agronomic characteristics and consistency of high vitamin C content, H06035-4 was selected as a high vitamin C content promising line.
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- 2013
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12. Possible anticipation associated with a novel splice site mutation in episodic ataxia type 2
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Ji Soo Kim, Jae-Hwan Choi, Dae Seong Kim, Min-Ji Kim, Ji Won Yook, Hyang-Sook Kim, Kwang Dong Choi, Young-Eun Park, and Jin Hong Shin
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Male ,Ataxia ,Genetic counseling ,DNA Mutational Analysis ,Dermatology ,Genetic analysis ,Nystagmus, Pathologic ,Young Adult ,Asian People ,Trinucleotide Repeats ,medicine ,Humans ,Age of Onset ,Child ,Aged ,Genetics ,Episodic ataxia ,Splice site mutation ,Anticipation, Genetic ,General Medicine ,Middle Aged ,medicine.disease ,Pedigree ,Psychiatry and Mental health ,Child, Preschool ,Mutation ,Mutation (genetic algorithm) ,Anticipation (genetics) ,Female ,Calcium Channels ,RNA Splice Sites ,Neurology (clinical) ,medicine.symptom ,Age of onset ,Psychology - Abstract
Anticipation is a phenomenon characterized by decreasing age at onset and increasing severity of symptoms of a disease in successive generations within a pedigree. Anticipation mostly occurs in neurodegenerative diseases with expansion of unstable trinucleotide repeats. However, it has not been previously pointed out in episodic ataxia type 2 (EA2). Clinical and genetic analyses were performed in nine members from three consecutive generations of a Korean family with EA2. We performed a polymerase chain reaction (PCR)-based direct sequence analysis of all coding regions of CACNA1A using genomic DNA. The clinically affected family members showed recurrent vertigo, interictal nystagmus, and childhood epilepsy. There is a decrease in the age onset (possible genetic anticipation) in three succeeding generations of the family. Genetic analysis identified a splice site mutation (p.Val1465Glyfs13X) and normal trinucleotide repeats in CACNA1A in all clinically affected and one unaffected members. Recognizing anticipation would aid in genetic counseling in EA2.
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- 2013
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13. Increased synovial expression of IL-27 by IL-17 in rheumatoid arthritis
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Geun Tae Kim, Chi Dae Kim, Seung-Hoon Baek, Young-Eun Park, So Youn Park, and Seung-Geun Lee
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Male ,musculoskeletal diseases ,Knee Joint ,Immunology ,Bone Marrow Cells ,Inflammation ,Arthritis, Rheumatoid ,Mice ,Immune system ,medicine ,Animals ,Humans ,Synovial fluid ,RNA, Messenger ,Interleukin 27 ,Pharmacology ,business.industry ,Interleukins ,Macrophages ,Cartilage ,Interleukin-17 ,Synovial Membrane ,Interleukin ,Dendritic Cells ,medicine.disease ,Arthritis, Experimental ,medicine.anatomical_structure ,Mice, Inbred DBA ,Rheumatoid arthritis ,Interleukin 17 ,medicine.symptom ,business - Abstract
Interleukin (IL) 17 plays an important role in synovial inflammation and bone destruction in rheumatoid arthritis (RA), while IL-27 exerts a regulatory role in T cell-mediated immune responses. Our aim was to study the influence of IL-17 on IL-27 production in RA. Following injection of IL-17 in the knee joint of CIA mice, synovium was examined for IL-17 and IL-27 expression by western blot, real-time PCR, and immunohistochemistry. IL-17 and IL-27 levels were measured by ELISA in mouse bone marrow-derived dendritic cells (BM-DCs) and in synovial fluid (SF) macrophages from RA patients. IL-17 exacerbated disease progression in CIA mice. Histological analysis showed increased pannus formation associated with cartilage and bone erosion following injection with IL-17. The expression of IL-27 was increased in CIA mice. The expression of IL-17 and IL-27 was increased more in IL-17-injected CIA mice than in control mice. The majority of cells expressing IL-27 were co-localized with synovial macrophages. Increased expression of IL-27 by application of recombinant IL-17 was confirmed in CIA BM-DCs and in SF macrophages from RA patients. IL-17 enhanced expression of IL-27 in synovial macrophages from RA patients and CIA mice, indicating an interaction between IL-17 and IL-27 as an autoregulatory mechanism.
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- 2012
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14. Expression of TLR2, TLR4, and TLR9 in dermatomyositis and polymyositis
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Young-Eun Park, Jun-Hee Lee, Mi-La Cho, Seung-Hoon Baek, Jung-Hee Kim, Wan Hee Yoo, Geun-Tae Kim, Ho-Youn Kim, Hye-Jwa Oh, Sung-Il Kim, Dae-Sung Kim, and Sun Hee Lee
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Adult ,CD4-Positive T-Lymphocytes ,Male ,Adolescent ,Biopsy ,Gene Expression ,Inflammation ,Polymyositis ,Dermatomyositis ,Interferon-gamma ,Young Adult ,Rheumatology ,immune system diseases ,parasitic diseases ,Humans ,Medicine ,Interferon gamma ,Muscle, Skeletal ,Interleukin 4 ,Aged ,Reverse Transcriptase Polymerase Chain Reaction ,Tumor Necrosis Factor-alpha ,business.industry ,Interleukin-17 ,hemic and immune systems ,General Medicine ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Toll-Like Receptor 2 ,Toll-like receptors ,Toll-Like Receptor 4 ,TLR2 ,Toll-Like Receptor 9 ,Immunology ,Original Article ,Female ,Tumor necrosis factor alpha ,Interleukin-4 ,Interleukin 17 ,medicine.symptom ,business ,medicine.drug - Abstract
The aim of this study was to investigate the expressions of Toll-like receptor (TLR) 2, TLR4, TLR9, and their correlations with the expression of cytokines that are associated with activation of CD4(+) T cells and inflammation including interferon gamma (IFNgamma), interleukin 4 (IL4), interleukin 17 (IL17), and tumor necrosis factor alpha (TNFalpha) in muscle tissues of patients with dermatomyositis (DM) and polymyositis (PM). The expressions of TLR2, TLR4, TLR9, IFNgamma, IL4, IL17, and TNFalpha were measured by real-time reverse transcription-polymerase chain reaction in muscle tissues from 14 patients with DM and PM (nine patients with DM, five patients with PM) and three controls. The expressions of TLR2, TLR4, and TLR9 were also localized with immunohistochemistry. The expression levels of TLR2, TLR4, TLR9, IFNgamma, IL4, IL17, and TNFalpha were significantly high in patients with DM and PM compared with those in the controls, and the expression levels of TLR4 and TLR9 had significant positive correlations with the expressions of IFNgamma, IL4, IL17, and TNFalpha. Immunohistochemistry showed that TLR2, TLR4, and TLR9 were expressed by infiltrating cells of perimysium in DM, whereas they were expressed by infiltrating cells of endomysium in PM. These results suggest that the involvement of TLR4 and TLR9 in immunopathogenesis of DM and PM might be connected with activation of CD4(+) T cells.
- Published
- 2009
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