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Background Obesity (waist circumference, body mass index (BMI)) and lifestyle factors (dietary habits, smoking, alcohol drinking, Sedentary behavior) have been associated with risk of benign prostatic hyperplasia (BPH) in observational studies, but whether these associations are causal is unclear. Methods We performed a univariable and multivariable Mendelian randomization study to evaluate these associations. Genetic instruments associated with exposures at the genome-wide significance level (P –8) were selected from corresponding genome-wide associations studies (n = 216,590 to 1,232,091 individuals). Summary-level data for BPH were obtained from the UK Biobank (14,126 cases and 169,762 non-cases) and FinnGen consortium (13,118 cases and 72,799 non-cases). Results from UK Biobank and FinnGen consortium were combined using fixed-effect meta-analysis. Results The combined odds ratios (ORs) of BPH were 1.24 (95% confidence interval (CI), 1.07–1.43, P = 0.0045), 1.08 (95% CI 1.01–1.17, P = 0.0175), 0.94 (95% CI 0.67–1.30, P = 0.6891), 1.29 (95% CI 0.88–1.89, P = 0.1922), 1.23 (95% CI 0.85–1.78, P = 0.2623), and 1.04 (95% CI 0.76–1.42, P = 0.8165) for one standard deviation (SD) increase in waist circumference, BMI, and relative carbohydrate, fat, protein and sugar intake, 1.05 (95% CI 0.92–1.20, P = 0.4581) for one SD increase in prevalence of smoking initiation, 1.10 (95% CI 0.96–1.26, P = 0.1725) and 0.84 (95% CI 0.69–1.02, P = 0.0741) for one SD increase of log-transformed smoking per day and drinks per week, and 1.31 (95% CI 1.08–1.58, P = 0.0051) for one SD increase in sedentary behavior. Genetically predicted waist circumference (OR = 1.26, 95% CI 1.11–1.43, P = 0.0004) and sedentary behavior (OR = 1.14, 95% CI 1.05–1.23, P = 0.0021) were associated with BPH after the adjustment of BMI. Conclusion This study supports independent causal roles of high waist circumference, BMI and sedentary behavior in BPH.

Background Known as the prerequisite component for the heterosis breeding system, the male sterile line determines the hybrid yield and seed purity. Therefore, a deep understanding of the mechanism and gene network that leads to male sterility is crucial. BS366, a temperature-sensitive genic male sterile (TGMS) line, is male sterile under cold conditions (12 °C with 12 h of daylight) but fertile under normal temperature (20 °C with 12 h of daylight). Results During meiosis, BS366 was defective in forming tetrads and dyads due to the abnormal cell plate. During pollen development, unusual vacuolated pollen that could not accumulate starch grains at the binucleate stage was also observed. Transcriptome analysis revealed that genes involved in the meiotic process, such as sister chromatid segregation and microtubule-based movement, were repressed, while genes involved in DNA and histone methylation were induced in BS366 under cold conditions. MethylRAD was used for reduced DNA methylation sequencing of BS366 spikes under both cold and control conditions. The differentially methylated sites (DMSs) located in the gene region were mainly involved in carbohydrate and fatty acid metabolism, lipid metabolism, and transport. Differentially expressed and methylated genes were mainly involved in cell division. Conclusions These results indicated that the methylation of genes involved in carbon metabolism or fatty acid metabolism might contribute to male sterility in BS366 spikes, providing novel insight into the molecular mechanism of wheat male sterility.

Energy dissipation rate is relevant in the turbulent phenomenology theory, such as the classical Kolmogorov 1941 and 1962 refined similarity hypothesis. However, it is extremely difficult to retrieve experimentally or numerically. In this paper, the full energy dissipation, its proxy and the pseudo-energy dissipation rate along the Lagrangian trajectories in the three-dimensional turbulent flows are examined by using a state-of-art high resolution direct numerical simulation database with a Reynolds number Re λ = 400. It is found that the energy dissipation proxy e P is more correlated with the full energy dissipation rate e. The corresponding correlation coefficient ρ between the velocity gradient and e shows a Gaussian distribution. Furthermore, the coarse-grained dissipation rate is considered. The cross correlation ρ is found to be increased with the increasing of the scale τ. Finally, the hierarchical structure is extracted for the full energy dissipation rate, its proxy and the pseudo one. The results show a power-law behavior in the inertial range 10 ≤τ/τ η ≤ 100. The experimental scaling exponent of the full energy dissipation rate is found to be h L =0.69, agrees very well with the one found for the Eulerian velocity. The experimental values for e P and e S are around h L = 0.78, implying a more intermittent Lagrangian turbulence. Therefore, the intermittency parameter provided by e P and e S will be biased.

Backgrounds Glioma is the most fatal primary brain glioma in central nervous system mainly attributed to its high invasion. Prucalopride, a Serotonin-4 (5-HT4) receptor agonist, has been reported to regulate neurodevelopment. This study aimed to investigate the influence of the Prucalopride on glioma cells and unveil underlying mechanism. Methods In this study, glioma cells proliferation was evaluated by Cell counting kit-8 (CCK8). Wound healing and transwell assay were used to test cellular migration and invasion. Flow cytometry was utilized to determine cellular apoptosis rate. Apoptosis related markers, autophagy markers, and protein kinase B (AKT)-mammalian target of rapamycin (mTOR) pathway key molecules were detected using western blot assay. Results As a result, the proliferation, migration and invasiveness of glioma cells were impaired by Prucalopride treatment, the apoptosis rate of glioma cells was enhanced by Prucalopride stimulation, accompanied by the increased pro-apoptosis proteins Bax and Cleaved caspase-3 and decreased anti-apoptosis protein Bcl-2. Prucalopride significantly promoted autophagy by increased expression level of Beclin 1 and LC3-II, while decreased expression level of p62. Prucalopride administration resulted in obvious inhibitions of key molecules of AKT-mTOR pathway, including phosphorylated- (p-) AKT, p-mTOR and phosphorylated-ribosomal p70S6 kinase (p-P70S6K). Conclusions Taking together, these results indicate that Prucalopride may be likely to play an anti-tumor role in glioma cells, which suggests potential implications for glioma promising therapy alternation in the further clinics.

The pinion bracket-assembly (PBA) is a major part of three gorges project (TGP) ship lift drive system. The static strength, fatigue strength and stress distribution of hinge pin of PBA were analyzed by ANSYS, and the structure of PBA was optimized. The results show that after the optimization, the maximum comprehensive stress is 259.59 MPa, the maximum fatigue cumulative damage of weld joints is 0.94 and the maximum vertical deformation of hinge pin is 0.14 mm. The elastic deformation, hydropneumatic spring cylinder (HSC) load response and the vibration characteristics of PBA were studied by the bearing test when PBA bore the load caused by different water level errors. The results indicate that when the water level of ship chamber ranges from 3.4 m to 3.6 m, the vertical elastic deformation of the pinion shaft is between −8.58 and 10.50 mm. When upward outage-load(1580 kN) is imposed by the test-rack, the vertical elastic deformation of the pinion shaft is 13.42 and 14.07 mm and HSC load response is 795.80–800.80 kN. In the process of imposing load on the pinion by the test-rack, the maximum vibration amplitude and acceleration of PBA internal components are 0.37° and 2.67 rad/s2, respectively; the maximum impact on the pin caused by vibration is 19.89 kN; the pinion shaft vertical displacement and HSC load response do not fluctuate. There is a great difference between the frequency of meshing force of the pinion and the rack (1.06 Hz) and first-order natural frequency of PBA(8.41 Hz), thus PBA will not resonate. From all above, PBA meets the static strength and fatigue strength requirements. The vibration of PBA internal components has no effect on the vertical displacement of the pinion shaft, HSC load response and smooth operation of PBA. There is a liner relationship in the ratio of 2:1 between the thrust imposed by the test-rack and HSC load, thus HSC can limit the load imposed on the pinion.

The Three Gorges Project (TGP) ship lift employs 4 safety mechanisms, of which one consists of a rotary locking screw and a nut jaw column. The thread pair clearance (TPC) of safety mechanisms is set at 60 mm. Owing to influential factors, the TPC changes randomly in the upward/downward-stroke of the ship chamber. If it diminished to 0, the safety mechanism would be jammed, thus resulting in disastrous accidents. By the bearing test of the drive system, 7 influential factors have been studied; 15 other influential factors (including 8 factors of manufacture and installation deviation, 3 factors of chamber offset, 2 factors of external load and 2 factors of wear) have been analyzed based on the design data. Results by the limit superposition reveal that the TPC change varies from −43.8 mm to +48.4 mm when the water level of the chamber ranges from 3.4 m to 3.6 m. According to the Gaussian distribution, the probability of the TPC change varied from −53.7 mm to +58.8 mm in the most detrimental status is 99.74%, therefore, the TPC remains in a safe condition. This paper puts forward that two-phase operation of the drive system should be adopted so as to reduce the maximum TPC change to −44.6 mm.