1. Control of cotton fibre elongation by a homeodomain transcription factor GhHOX3
- Author
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Hua Yu Zhu, Xiao-Ya Chen, Yan Da Zeng, Xueying Guan, Z. Jeffrey Chen, Tianzhen Zhang, Bao Liang Zhou, Ling-Jian Wang, Chang-Qing Yang, Lumen Chao, Xiao Xia Shangguan, Guanjing Hu, Wang Zhen Guo, Jonathan F. Wendel, Xiu Fang Zhang, Chun-Min Shan, and Bo Zhao
- Subjects
Leucine zipper ,Molecular Sequence Data ,Quantitative Trait Loci ,General Physics and Astronomy ,Repressor ,Biology ,Bioinformatics ,Histone Deacetylases ,General Biochemistry, Genetics and Molecular Biology ,Plant Growth Regulators ,Gene Expression Regulation, Plant ,Transcription (biology) ,Cotton Fiber ,Gene ,Transcription factor ,Plant Proteins ,Homeodomain Proteins ,Regulation of gene expression ,Gossypium ,Leucine Zippers ,Multidisciplinary ,Gene Expression Profiling ,Gene Expression Regulation, Developmental ,food and beverages ,Trichomes ,General Chemistry ,Gibberellins ,Cell biology ,Fiber cell ,Homeobox - Abstract
Cotton fibres are unusually long, single-celled epidermal seed trichomes and a model for plant cell growth, but little is known about the regulation of fibre cell elongation. Here we report that a homeodomain-leucine zipper (HD-ZIP) transcription factor, GhHOX3, controls cotton fibre elongation. GhHOX3 genes are localized to the 12th homoeologous chromosome set of allotetraploid cotton cultivars, associated with quantitative trait loci (QTLs) for fibre length. Silencing of GhHOX3 greatly reduces (>80%) fibre length, whereas its overexpression leads to longer fibre. Combined transcriptomic and biochemical analyses identify target genes of GhHOX3 that also contain the L1-box cis-element, including two cell wall loosening protein genes GhRDL1 and GhEXPA1. GhHOX3 interacts with GhHD1, another homeodomain protein, resulting in enhanced transcriptional activity, and with cotton DELLA, GhSLR1, repressor of the growth hormone gibberellin (GA). GhSLR1 interferes with the GhHOX3–GhHD1 interaction and represses target gene transcription. Our results uncover a novel mechanism whereby a homeodomain protein transduces GA signal to promote fibre cell elongation.
- Published
- 2014