10 results on '"Wenping Xu"'
Search Results
2. Joint multi-view character embedding model for named entity recognition of Chinese car reviews
- Author
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Jiaming Ding, Wenping Xu, Anning Wang, Shuangyao Zhao, and Qiang Zhang
- Subjects
Artificial Intelligence ,Software - Published
- 2023
3. Bifenthrin induces DNA damage and autophagy in Spodoptera frugiperda (Sf9) insect cells
- Author
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Miaoqing Lu, Yang Zhang, Yun Yang, Wenping Xu, Lianhua Zhu, Liming Tao, and Zhiping Xu
- Subjects
0301 basic medicine ,Cell Survival ,DNA damage ,Bifenthrin ,Sf9 ,Spodoptera ,Inhibitory Concentration 50 ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Downregulation and upregulation ,Western blot ,Pyrethrins ,Autophagy ,Sf9 Cells ,medicine ,Animals ,Cytotoxic T cell ,biology ,medicine.diagnostic_test ,fungi ,Cell Biology ,General Medicine ,biology.organism_classification ,Mitochondria ,Cell biology ,030104 developmental biology ,chemistry ,Cell culture ,030220 oncology & carcinogenesis ,Reactive Oxygen Species ,DNA Damage ,Developmental Biology - Abstract
Bifenthrin is one of the most commonly used synthetic pyrethroid insecticides. It targets the nervous system of insects, mainly acting on sodium channels in nerve cell membranes. The high use of bifrenthrin may lead to an increase in pest insect resistance. Additionally, there are only a few studies describing its cytotoxic action. A series of bioassays were carried out, and the results showed that bifenthrin has a significant ability to induce DNA damage and the inhibition of viability in Spodoptera frugiperda (Sf9) cells. Monodansylcadaverine staining and transmission electron microscope assays were used to observe significant levels of autophagosomes and mitochondrial dysfunction in the cytoplasm. Additionally, western blot analysis showed an upregulation in LC3-II and beclin-1 protein expression and a downregulation in p62 expression, which contributed to the cytotoxic effect of bifenthrin on Sf9 cells. Overall, bifenthrin significantly impacts the viability of Sf9 cells by inducing DNA damage and autophagy. These results provide a theoretical basis for understanding bifrenthin's mechanism of cytotoxicity.
- Published
- 2021
4. The different effects of natural pyrethrins and beta-cypermethrin on human hepatocyte QSG7701 cells by ROS-mediated oxidative damage
- Author
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Liming Tao, Yun Yang, Wenping Xu, Wenjing Liu, Jian Wang, and Yang Zhang
- Subjects
0301 basic medicine ,Insecticides ,DNA damage ,Health, Toxicology and Mutagenesis ,010501 environmental sciences ,Pharmacology ,01 natural sciences ,Cell Line ,Inhibitory Concentration 50 ,03 medical and health sciences ,chemistry.chemical_compound ,Pyrethrins ,Pyrethrin ,Humans ,Environmental Chemistry ,Bioassay ,Cytotoxic T cell ,Cytotoxicity ,IC50 ,Cell Proliferation ,0105 earth and related environmental sciences ,Membrane Potential, Mitochondrial ,Dose-Response Relationship, Drug ,Chemistry ,General Medicine ,Pollution ,Chromatin ,In vitro ,Oxidative Stress ,030104 developmental biology ,Toxicity ,Hepatocytes ,Comet Assay ,Reactive Oxygen Species ,DNA Damage - Abstract
With the widespread use of natural pyrethrins and pyrethroids to defend pest insects, people had the sustained interest in the potential risk to environment and human health. However, the research about natural pyrethrins and beta-cypermethrin induction of cytotoxicity is still seldom. This study is about the cytotoxic effects of these on human non-target cells in vitro. The cytotoxic effect of natural pyrethrins and beta-cypermethrin on QSG7701 cells were researched by using various bioassays in vitro. The results suggested that with the natural pyrethrin concentration increased, the viability of QSG7701 cells were inhibited increasingly, and the IC50 value as calculated was approximately 42.54 and 18.68 μg/mL after the cells were treated 24 and 48 h. The proliferative potential of QSG7701 cells treated with 40 μg/mL natural pyrethrins 6 and 12 h was decreased by 67.44 and 94.74%, dramatic enhancement ROS, collapse of mitochondrial membrane potential, DNA exhibit severity of impairment, and chromatin DNA condensation. However, beta-cypermethrin has lower toxicity than natural pyrethrins. The IC50 values of beta-cypermethrin were all > 80 μg/mL, and the colony formation expression was decreased by 15.26 and 19.09%, which implied that natural pyrethrins are more significantly cytotoxic and potentially genotoxic to human hepatocyte cells.
- Published
- 2018
5. Selection of reference genes for miRNA qRT-PCR under abiotic stress in grapevine
- Author
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Caixi Zhang, Hui Li, Shiren Song, Meng Luo, Zhen Gao, Chao Ma, Wenping Xu, Qin Li, and Shiping Wang
- Subjects
0106 biological sciences ,0301 basic medicine ,Salinity ,lcsh:Medicine ,Biology ,Real-Time Polymerase Chain Reaction ,01 natural sciences ,Article ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Stress, Physiological ,Reference genes ,microRNA ,Vitis ,lcsh:Science ,Gene ,Plant Proteins ,Abiotic component ,Regulation of gene expression ,Genetics ,Multidisciplinary ,Abiotic stress ,Gene Expression Profiling ,lcsh:R ,fungi ,Reference Standards ,Droughts ,Cold Temperature ,MicroRNAs ,030104 developmental biology ,Real-time polymerase chain reaction ,RNA, Plant ,lcsh:Q ,Algorithms ,010606 plant biology & botany - Abstract
Grapevine is among the fruit crops with high economic value, and because of the economic losses caused by abiotic stresses, the stress resistance of Vitis vinifera has become an increasingly important research area. Among the mechanisms responding to environmental stresses, the role of miRNA has received much attention recently. qRT-PCR is a powerful method for miRNA quantitation, but the accuracy of the method strongly depends on the appropriate reference genes. To determine the most suitable reference genes for grapevine miRNA qRT-PCR, 15 genes were chosen as candidate reference genes. After eliminating 6 candidate reference genes with unsatisfactory amplification efficiency, the expression stability of the remaining candidate reference genes under salinity, cold and drought was analysed using four algorithms, geNorm, NormFinder, deltaCt and Bestkeeper. The results indicated that U6 snRNA was the most suitable reference gene under salinity and cold stresses; whereas miR168 was the best for drought stress. The best reference gene sets for salinity, cold and drought stresses were miR160e + miR164a, miR160e + miR168 and ACT + UBQ + GAPDH, respectively. The selected reference genes or gene sets were verified using miR319 or miR408 as the target gene.
- Published
- 2018
6. Evaluation of Immunosuppressive Activity of Demethylzeylasteral in a Beagle Dog Kidney Transplantation Model
- Author
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Jianping Zhang, Huimin An, Yu Zhu, Zongming Lin, Wenping Xu, and Yujun Liu
- Subjects
Graft Rejection ,Male ,medicine.medical_specialty ,medicine.medical_treatment ,Biophysics ,Pharmacology ,Biochemistry ,Beagle ,Organ transplantation ,Dogs ,medicine ,Animals ,Inhibitory effect ,Kidney transplantation ,Immune rejection ,Immunosuppression Therapy ,biology ,business.industry ,Immunosuppression ,Cell Biology ,General Medicine ,Demethylzeylasteral ,medicine.disease ,biology.organism_classification ,Kidney Transplantation ,Triterpenes ,Cyclosporine ,Tripterygium wilfordii ,business ,Immunosuppressive Agents - Abstract
Several monomers isolated from Tripterygium wilfordii Hook f. (Celastraceae) have attracted worldwide interest. In this study, we established a simple and reliable kidney transplantation model in beagle dog to evaluate the immunosuppressive activity of demethylzeylasteral (T-96), an immunosuppressive monomer isolated from the root xylem of T. wilfordii. Recipient and donor male beagle dogs were obtained from two different breeders to ensure MHC mismatching. All dogs were randomly divided into six groups following kidney transplantation, and different doses of T-96 or cyclosporine A (CsA) were administered to each group during 14 days of observation. The results showed that T-96 alone at a dosage of 10 or 20 mg/kg/day prolonged graft survival up to 10.83 ± 1.47 or 11.17 ± 1.47 days. A combination of T-96 and CsA significantly prolonged the survival time to 13.33 ± 1.75 days. The results demonstrated that T-96 can inhibit acute rejection in kidney transplantation, and the inhibitory effect of T-96 was enhanced when combined with CsA, which suggests the possible use in organ transplantation to prevent immune rejection.
- Published
- 2015
7. Anordrin Eliminates Tamoxifen Side Effects without Changing Its Antitumor Activity
- Author
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Shuangjie Wang, Jian Wang, Wenping Xu, Chengshui Chen, Aijie Xin, Long Yang, Jun Yang, Wenwen Gu, Bubing Zeng, Xu Zhang, Nian Dong, Guowu Chen, Xiaoxi Sun, and Zhong Ni
- Subjects
0301 basic medicine ,Antineoplastic Agents, Hormonal ,medicine.drug_class ,Mice, Nude ,Estrogen receptor ,Pharmacology ,Article ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Non-alcoholic Fatty Liver Disease ,Cell Line, Tumor ,Animals ,Humans ,Medicine ,skin and connective tissue diseases ,Active metabolite ,Mice, Inbred ICR ,Norandrostanes ,Multidisciplinary ,business.industry ,Fatty liver ,medicine.disease ,Contraceptives, Oral, Synthetic ,Epithelium ,Endometrial Neoplasms ,Tamoxifen ,Treatment Outcome ,030104 developmental biology ,medicine.anatomical_structure ,Estrogen ,Cell culture ,030220 oncology & carcinogenesis ,Heterografts ,Female ,business ,Neoplasm Transplantation ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
Tamoxifen is administered for estrogen receptor positive (ER+) breast cancers, but it can induce uterine endometrial cancer and non-alcoholic fatty liver disease (NAFLD). Importantly, ten years of tamoxifen treatment has greater protective effect against ER+ breast cancer than five years of such treatment. Tamoxifen was also approved by the FDA as a chemopreventive agent for those deemed at high risk for the development of breast cancer. The side effects are of substantial concern because of these extended methods of tamoxifen administration. In this study, we found that anordrin, marketed as an antifertility medicine in China, inhibited tamoxifen-induced endometrial epithelial cell mitosis and NAFLD in mouse uterus and liver as an anti-estrogenic and estrogenic agent, respectively. Additionally, compared with tamoxifen, anordiol, the active metabolite of anordrin, weakly bound to the ligand binding domain of ER-α. Anordrin did not regulate the classic estrogen nuclear pathway; thus, it did not affect the anti-tumor activity of tamoxifen in nude mice. Taken together, these data suggested that anordrin could eliminate the side effects of tamoxifen without affecting its anti-tumor activity.
- Published
- 2017
8. Root restriction affected anthocyanin composition and up-regulated the transcription of their biosynthetic genes during berry development in ‘Summer Black’ grape
- Author
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Xiuming Yu, Caixi Zhang, Jianjun He, Bo Wang, Bai Xianjin, Jiefa Li, Shiping Wang, Wenping Xu, Yang Bai, and Cao Xiongjun
- Subjects
chemistry.chemical_classification ,Chalcone isomerase ,Chalcone synthase ,biology ,Physiology ,Chemistry ,fungi ,Flavonoid ,food and beverages ,Plant Science ,Glutathione ,Berry ,carbohydrates (lipids) ,chemistry.chemical_compound ,Biochemistry ,Anthocyanin ,Gene expression ,biology.protein ,Agronomy and Crop Science ,Flavanone - Abstract
Root restriction was applied to ‘Summer black’ grape (Vitis vinifera L. × Vitis labrusca L.) to investigate its effect on anthocyanin biosynthesis in grape berry during development. Anthocyanin composition and expression patterns of 16 genes in anthocyanin pathway were thus analyzed. The results showed that the anthocyanin levels in berry skin were significantly increased and the anthocyanin profile was enriched. Gene expression pattern revealed that the increased anthocyanins coincide with the up-regulated expression of all 16 genes investigated, including phenylalanine ammonia-lyase, 4-coumarate CoA ligase, chalcone synthase 1, chalcone synthase 2, chalcone synthase 3, chalcone isomerase, flavanone 3-hydroxylase 1, flavanone 3-hydroxylase 2, flavonoid 3′-hydroxylase (F3′H), flavonoid 3′,5′-hydroxylase (F3′5′H), di-hydroflavonol 4-reductase, leucoanthocyanidin dioxygenase, O-methyltransferases (OMT), UDP-glucose:flavonoid 3-O-glucosyl-transferase (3GT), UDP-glucose:flavonoid 5-O-glucosyl-transferase (5GT) and glutathione S-transferase (GST). The increased total anthocyanins predominantly resulted from the increase of tri-hydroxylated, methoxylated and mono-glycosylated rather than di-hydroxylated, non-methoxylated, and di-glycosylated forms, which might be due to the differential regulation of F3′5′H/F3′H, OMT and 3GT, respectively.
- Published
- 2013
9. Hydroxylation of thiacloprid by bacterium Stenotrophomonas maltophilia CGMCC1.1788
- Author
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Jueping Ni, Cigang Yu, Jun Luo, Sheng Yuan, Yin-Juan Zhao, Wenping Xu, and Yi-Jun Dai
- Subjects
Magnetic Resonance Spectroscopy ,Time Factors ,Environmental Engineering ,Piperonyl Butoxide ,Pyridines ,Stereochemistry ,Stenotrophomonas maltophilia ,Thiazines ,Bioengineering ,Whitefly ,Hydroxylation ,Microbiology ,Acetamiprid ,Neonicotinoids ,chemistry.chemical_compound ,Imidacloprid ,Animals ,Environmental Chemistry ,Biotransformation ,Chromatography, High Pressure Liquid ,biology ,Imidazoles ,Neonicotinoid ,Nitro Compounds ,biology.organism_classification ,Thiacloprid ,Pollution ,Solutions ,Nitroguanidine ,chemistry ,Aphids ,Biological Assay - Abstract
Chloropyridinyl neonicotinoid insecticides play a major role in crop protection and flea control on cats and dogs. Imidacloprid, thiacloprid and acetamiprid have in common the 6-chloro-3-pyridinylmethyl group but differ in the nitroguanidine or cyanoamidine substituent on an acyclic or cyclic moiety. Our previous study found that Stenotrophomonas maltophilia CGMCC 1.1788 could hydroxylate imidacloprid to 5-hydroxy imidacloprid, and 5-hydroxy imidacloprid was easily converted to 10-19 times higher insecticidal olefin imidacloprid against aphid or whitefly. Acetamiprid could be transformed by S. maltophilia to form N-demethylation product(IM 2-1). In this paper, we examined S. maltophilia CGMCC 1.1788's ability of transformation of thiacloprid. S. maltophilia CGMCC 1.1788 can hydroxylate thiacloprid to 4-hydroxy thiacloprid characterized by HPLC-MS/MS and NMR analysis, however 4-hydroxy thiacloprid could not be converted to olefin thiacloprid under acid conditions like imidacloprid, whereas oxidized and decyonated simultaneously to form 4-ketone thiacloprid imine in alkaline solution. Bioassays indicated that 4-hydroxy thiacloprid had 156 times lower insecticidal activity than thiacloprid, and the ketone-imine derivative almost had no toxicity towards aphid. Though both imidacloprid and thiacloprid are hydroxylated by S. maltophilia CGMCC 1.1788 at the same carbon atom position, however the structural difference between in imidacloprid and thiacloprid, originate the entire discrepancy in bioefficacy of metabolite and its further degrading pathway. These results explain that why thiacloprid is classified as not relevant grade for soil and seed applications, whereas imidacloprid is recommended and acetamiprid is limited.
- Published
- 2009
10. Antifungal Macrolides from Streptomyces cavourensis YY01-17
- Author
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Cong-cong Si, Liming Tao, Daojing Zhang, and Wenping Xu
- Subjects
Antifungal ,Streptomyces cavourensis ,medicine.drug_class ,Chemistry ,Botany ,medicine ,Plant Science ,General Chemistry ,General Biochemistry, Genetics and Molecular Biology - Published
- 2013
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