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The development of optical organic nanoparticles (NPs) is desirable and widely studied. However, most organic dyes are water-insoluble such that the derivatization and modification of these dyes are difficult. Herein, we demonstrated a simple platform for the fabrication of organic NPs designed with emissive properties by loading ten different organic dyes (molar masses of 479.1–1081.7 g/mol) into water-soluble polymer nanosponges composed of poly(styrene-alt-maleic acid) (PSMA). The result showed a substantial improvement over the loading of commercial dyes (3.7–50% loading) while preventing their spontaneous aggregation in aqueous solutions. This packaging strategy includes our newly synthesized organic dyes (> 85% loading) designed for OPVs (242), DSSCs (YI-1, YI-3, YI-8), and OLEDs (ADF-1–3, and DTDPTID) applications. These low-cytotoxicity organic NPs exhibited tunable fluorescence from visible to near-infrared (NIR) emission for cellular imaging and biological tracking in vivo. Moreover, PSMA NPs loaded with designed NIR-dyes were fabricated, and photodynamic therapy with these dye-loaded PSMA NPs for the photolysis of cancer cells was achieved when coupled with 808 nm laser excitation. Indeed, our work demonstrates a facile approach for increasing the biocompatibility and stability of organic dyes by loading them into water-soluble polymer-based carriers, providing a new perspective of organic optoelectronic materials in biomedical theranostic applications.

Efficient red emissive carbon dots (CDs) in aqueous solutions are very scarce for high performance bioimaging applications. In this work, we report a one-step solvothermal treatment to synthesize pure red emissive CDs (FA-CDs) from citric acid and urea in formic acid without complicated purification procedures. Photoluminescence quantum yield (PLQY) of 43.4% was observed in their dimethyl sulfoxide solutions. High PLQY up to 21.9% in aqueous solutions was achieved in their bovine serum albumin (BSA) composites (FA-CDs@BSA) with significantly enhanced multi-photon fluorescence. The strong surface electron-withdrawing structure of FA-CDs caused by the high content of C = O groups contributes for their pure red emission. Owing to the significantly enhanced single and multi-photon red fluorescence and enlarged particle sizes after composing with BSA, in vivo tumor imaging and two-photon fluorescence imaging of blood vessels in mouse ear have been realized via intravenous injection of FA-CDs@BSA aqueous solutions.

We investigated the impact of ecological conservation volunteer tourism on aboriginal culture and ecological conservation. We present herein a case of green sea turtle conservation volunteer tourism at Orchid Island, Taiwan. We used field observations and in-depth interviews with semi-structured and open-ended questionnaires to collect data. Data and method triangulation approaches were adopted to ensure the reliability of the results. We presented that cultural changes resulting from volunteer tourism threaten the survival of endangered species. In addition, the failure of the market incentive mechanism and dysfunctional internal communication within the conservation organization, two issues that have not been reported in previous studies, can guide future studies on volunteer tourism and ecological conservation. We also provided practical recommendations for Orchid Island green sea turtle conservation, i.e., the combination of traditional and modern ecological methods, and the consideration of local norms and taboos during organizational planning. The planning and implementing of conservation activity that respects local customs fosters the support of the local people. Finally, implementing the locals’ initial ecological conservation mechanisms can prevent the conservation activity from causing an undesirable threat to the conserved species.

Climate change can have a serious impact on water resources. The main agricultural product in southern Taiwan is rice, the planting of which consumes far more water than other crops. This makes agriculture in Taiwan especially vulnerable to climate change. In this study, we used the generalized watershed loading functions (GWLF) hydrological model to simulate the discharge of the Kaoping River under climate change scenarios A2 and B2 as released by the Intergovernmental Panel on Climate Change. We discussed the potential impact of climate change on water resources based on the results of GWLF simulations carried out using rainfall and temperature data from five general circulation models (GCMs). The simulation results indicate that river discharge in the wet season increases significantly, and decreases in the dry season. The discharge variations from using the various GCMs as inputs fall within the range of −26 to +15 % for the dry season and −10 to +82 % for the wet season. The variation in available water will seriously impact the first period rice farming (the period between the beginning of January and the end of May) in southern Taiwan. Consequently, effective reduction in conveyance loss in the irrigation canal systems and proper fallowing of paddy fields will be the main challenges to Taiwan’s agricultural sector for alleviating the impact of climate change. For further decision making, we show the effects of adapting to climate change by various degrees of the following two methods: fallowing paddy fields to various degrees and reducing conveyance loss in irrigation canal systems.

Virus is known to resonate in the confined-acoustic dipolar mode with microwave of the same frequency. However this effect was not considered in previous virus-microwave interaction studies and microwave-based virus epidemic prevention. Here we show that this structure-resonant energy transfer effect from microwaves to virus can be efficient enough so that airborne virus was inactivated with reasonable microwave power density safe for the open public. We demonstrate this effect by measuring the residual viral infectivity of influenza A virus after illuminating microwaves with different frequencies and powers. We also established a theoretical model to estimate the microwaves power threshold for virus inactivation and good agreement with experiments was obtained. Such structure-resonant energy transfer induced inactivation is mainly through physically fracturing the virus structure, which was confirmed by real-time reverse transcription polymerase chain reaction. These results provide a pathway toward establishing a new epidemic prevention strategy in open public for airborne virus.

Apoptosis or programmed cell death is the result of complex biochemical and genetic signals triggered by normal physiological events or by exposure to toxic compounds. Apoptosis can be revealed early in cells and tissues due to morphological and biochemical alterations of the cell. Here, we review recent progress in the study of the induction and detection of apoptosis in zooplankton copepods after their exposure to different apoptotic-inducer compounds, and we propose methods to predict copepod mortality even when animals appear normal. We also discuss the use of higher harmonic generation microscopy and multiphoton fluorescence microscopy techniques as possible tools to visualize apoptosis in vivo copepods without using any fluorescent probes.

The assessment to vulnerability of the water supply system needs several modeling tools and cases to simulate. A decision-support tool which integrates the common procedures of impact assessment of climate change, downscaling, weather generation, hydrological model, and interface for linking system dynamics model is proposed here. In this study, the impact of climatic change and growing water demand to the water supply system in Touchien river basin in Taiwan was derived. The vulnerability for the current water supply system was estimated for present and future conditions. The result demonstrated that the water supply system could meet the water demand in Touchien river basin but might be subjected to serious water shortage due to climatic change and expanded water demand. Results are expected to give the authorized government sectors a hint for instituting water policy and implementing allocation measures for irrigational water.

Using in vivo second harmonic generation (SHG) and third harmonic generation (THG) microscopies, we tracked the course of collagen remodeling over time in the same melanoma microenvironment within an individual mouse. The corresponding structural and morphological changes were quantitatively analyzed without labeling using an orientation index (OI), the gray level co-occurrence matrix (GLCM) method and the intensity ratio of THG to SHG (RTHG/SHG). In the early stage of melanoma development, we found that collagen fibers adjacent to a melanoma have increased OI values and SHG intensities. In the late stages, these collagen networks have more directionality and less homogeneity. The corresponding GLCM traces showed oscillation features and the sum of squared fluctuation VarGLCM increased with the tumor sizes. In addition, the THG intensities of the extracellular matrices increased, indicating an enhanced optical inhomogeneity. Multiplying OI, VarGLCM and RTHG/SHG together, the combinational collagen remodeling (CR) index at 4 weeks post melanoma implantation showed a 400-times higher value than normal ones. These results validate that our quantitative indices of SHG and THG microscopies are sensitive enough to diagnose the collagen remodeling in vivo. We believe these indices have the potential to help the diagnosis of skin cancers in clinical practice.

Plasmonic photothermal therapy (PPTT) using plasmonic nanoparticles as efficient photoabsorbing agents has been proposed previously. One critical step in PPTT is to effectively deliver gold nanoparticles into the cells. This study demonstrates that the delivery of gold nanorods (AuNRs) can be greatly enhanced by combining the following three mechanisms: AuNRs encapsulated in protein-shell microbubbles (AuMBs), molecular targeting, and sonoporation employing acoustic cavitation of microbubbles (MBs). Both in vitro and in vivo tests were performed. For molecular targeting, the AuMBs were modified with anti-VEGFR2. Once bound to the angiogenesis markers, the MBs were destroyed by ultrasound to release the AuNRs and the release was confirmed by photoacoustic measurements. Additionally, acoustic cavitation was induced during MB destruction for sonoporation (i.e., increase in transient cellular permeability). The measured inertial cavitation dose was positively correlated with the temperature increase at the tumor site. The quantity of AuNRs delivered into the cells was also determined by measuring the mass spectrometry and observed using third-harmonic-generation microscopy and two-photon fluorescence microscopy. A temperature increase of 20 °C was achieved in vitro. The PPTT results in vivo also demonstrated that the temperature increase (45 °C) provided a sufficiently high degree of hyperthermia. Therefore, synergistic delivery of AuNRs was demonstrated.

With the development of nonlinear optics and new imaging methods, near-infrared (NIR) light can excite contrast agents to probe biological specimens both functionally and structurally with a deeper imaging depth and a higher spatial resolution than linear optical approaches. There is considerable and growing interest in how biological specimens respond to NIR light. Moreover, the visible absorption band of most functional nanomaterials becomes NIR-excitable through multiphoton processes, thus allowing multifunctional imaging and combined therapy with noble metal and magnetic nanoparticles both in vitro and in vivo. A groundbreaking example is the use of different laser techniques to excite single-type NIR-absorbing/emitting nanomaterials to produce multiphoton emission by femtosecond lasers using either a remote control system for photodynamic therapy or photo-induced chemical bond dissociation. These techniques provided superior anatomical resolution and detection sensitivity for in vivo tumor-targeted imaging than those offered by conventional methods. Here we summarize the most recent progress in the development of smart NIR-absorbing/emitting nanomaterials for in vivo bioapplications. Nanomaterials that absorb or emit near-infrared light are increasingly being used to probe the structures and functions of biosystems. In particular, they are valuable for in vivo monitoring of drug nanocarriers, which offer many advantages over conventional drug-delivery methods. Joao Conde of Massachusetts Institute of Technology in the USA and co-workers give a timely review of recent progress in this area. They consider the various types of near-infrared absorbing and emitting nanomaterials. The scientists assess nine kinds of nanoparticles that are useful for multiphoton microscopy. They then overview toxicity studies of these materials. Finally, the researchers provide a roadmap for future research by listing ten properties that ideal near-infrared bioprobes would possess. Specifically, they note that ideal bioprobes should be biocompatible, versatile and multifunctional. A huge interest shows that optical contrast agents to probe biological specimen both functionally and structurally with deeper action depth and better spatial resolution is closely related with the development of near-infrared (NIR) light excitation and nonlinear optics and new imaging methods. This review will go beyond the state-of-the-art by revisiting the up-to-date progress in developing smart NIR-to-NIR and upconverted nanomaterials for in vivo bioapplications. The latest advances in the development of novel NIR-to-NIR linear and nonlinear optical nanomaterials and their potential applications in cancer targeting, diagnosis and therapeutics and deep-tissue imaging are described.