1. Durable targeting of B-lymphocytes in living mice
- Author
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L. Stein, Daniel Huynh, J. Decker, Thomas M. Lanigan, Marilia Cascalho, Lonnie D. Shea, Jeffrey L. Platt, and Adam R. Lefferts
- Subjects
Male ,0301 basic medicine ,Science ,Cellular differentiation ,Antigens, CD19 ,Genetic Vectors ,Spleen ,Article ,Statistics, Nonparametric ,CD19 ,Flow cytometry ,Green fluorescent protein ,Antibodies, Monoclonal, Murine-Derived ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Antigen ,Genes, Reporter ,Transduction, Genetic ,medicine ,Animals ,Luciferases ,Cell Proliferation ,B-Lymphocytes ,Reporter gene ,Luminescent Agents ,Multidisciplinary ,medicine.diagnostic_test ,biology ,Lentivirus ,Cell Differentiation ,Flow Cytometry ,3. Good health ,Cell biology ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,biology.protein ,Medicine ,Female ,Antibody ,030215 immunology - Abstract
Transfer to and enduring expression of genes in B cells has proved a vexing challenge. We report here a novel method for the specific and durable targeting of B lymphocytes in living mice. The method involves generation of lentiviruses pseudotyped with an anti-CD19 antibody. CD19 targeting viruses injected in the spleen of living mice efficiently transduced B cells and plasma cells detected by flow cytometry analysis of GFP expression. Expression of the reporter gene could be detected in the intact animal by external imaging for more than a year and was enhanced by booster immunization. Our method thus enables the specific delivery, expression and localization by external imaging of exogenous genes to the B cells and plasma cells of living individuals.
- Published
- 2018